Detection of Human Papillomavirus Type 16 DNA in Head and Neck Tumors

YANG Ping; DIAO Wen-Xian; LIU Xue-Feng; WU Xin-Xing; DING Xiao-Hua; YAO Chang-De; YANG Qiang

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June 1992

YANG Ping, DIAO Wen-Xian, LIU Xue-Feng, WU Xin-Xing, DING Xiao-Hua, YAO Chang-De and YANG Qiang. Detection of Human Papillomavirus Type 16 DNA in Head and Neck Tumors[J]. Virologica Sinica, 1992, 7(3).

Citation: YANG Ping, DIAO Wen-Xian, LIU Xue-Feng, WU Xin-Xing, DING Xiao-Hua, YAO Chang-De, YANG Qiang. Detection of Human Papillomavirus Type 16 DNA in Head and Neck Tumors .VIROLOGICA SINICA, 1992, 7(3) : 259.

头颈部肿瘤基因组中HPV16DNA的同源序列

1.
湖北医学院病毒研究所
2.
湖北医学院第二附属和
3.
第一附属医院武汉

摘要

利用头颈部肿癌临床活检新鲜组织和石蜡包埋组织的两种处理标本,分别采用核酸分子杂交和聚合酶链反应(PCR)两种技术,分析了头颈部肿瘤组织基因组中人乳头瘤病毒16型的同源序列,并研究HPV16的感染同头颈部肿瘤发生发展的关系。结果表明:1.喉鳞状细胞癌DNA中有HPV16 DNA同源序列,检测频率在20.0％以上。2.PCR扩增石蜡包埋肿瘤组织DNA中HPV16的URR(病毒基因上游调控区)中的序列,电泳分析扩增产物在喉乳头状瘤、喉癌、鼻腔内翻性乳头瘤和口腔癌中检测率分别是11.1％、20.0％、42.9％和27.3％。3.研究表明HPV16 DNA阳性率与喉癌原发部位,分化程度和临床分期之间可能有一定的相关性。人乳头瘤病毒可能是头颈部肿瘤的病毒病因之
- 头颈部肿瘤
- , 人乳头瘤病毒
- , 聚合酶链反应
- , 上游调控区
- , 基因扩增

Detection of Human Papillomavirus Type 16 DNA in Head and Neck Tumors

1.
Virus Research Institute
2.
Hubei Medical College
3.
Wuhan 430071

Abstract

In order to probe the relatignship between human papillonavirus type 16 and head-neck tumors, using fresh tissue and paraffin-embeded tissue sections preparing DNA to detect HPV16 DNA in head and neck tumor, by PCR and hybridization technique. The results showed that HPV16 DNA sequences were positively amplified in one laryngeal papilloma (11.1％), in 3 inverted papillomas of the nose (42.9％). in 4 laryngeal squamous cell earcinomas(20％).in 3 oral eaneers(27.3％). LSCC of glottie origin showed higher PHV16 DN...
- Head and neck neoplasm Human papillomaviruses Polymerase chain reaetion（PCR） Upsteam regulatory region（URR） Gene amplification

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Detection of Human Papillomavirus Type 16 DNA in Head and Neck Tumors

1. Virus Research Institute
2. Hubei Medical College
3. Wuhan 430071

Abstract: In order to probe the relatignship between human papillonavirus type 16 and head-neck tumors, using fresh tissue and paraffin-embeded tissue sections preparing DNA to detect HPV16 DNA in head and neck tumor, by PCR and hybridization technique. The results showed that HPV16 DNA sequences were positively amplified in one laryngeal papilloma (11.1％), in 3 inverted papillomas of the nose (42.9％). in 4 laryngeal squamous cell earcinomas(20％).in 3 oral eaneers(27.3％). LSCC of glottie origin showed higher PHV16 DN...