Restriction Enzyme Analysis and Molecular Cloning of CAV-1 DNA

BAI Li-Ping; TONG Guang-Zhi; DIAO Yong-Jun; TUN Bao-Cheng; YUE Jun-Meng; WANG Mei; YAN Shen

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June 1992

BAI Li-Ping, TONG Guang-Zhi, DIAO Yong-Jun, TUN Bao-Cheng, YUE Jun-Meng, WANG Mei and YAN Shen. Restriction Enzyme Analysis and Molecular Cloning of CAV-1 DNA[J]. Virologica Sinica, 1992, 7(3).

Citation: BAI Li-Ping, TONG Guang-Zhi, DIAO Yong-Jun, TUN Bao-Cheng, YUE Jun-Meng, WANG Mei, YAN Shen. Restriction Enzyme Analysis and Molecular Cloning of CAV-1 DNA .VIROLOGICA SINICA, 1992, 7(3) : 357.

犬Ⅰ型腺病毒DNA的酶切分析及分子克隆

1.
中国农科院哈尔滨兽医研究所国家兽医生物技术实验室
2.
解放军兽医大学军事兽医研究所
3.
解放军兽医大学军事兽医研究所哈尔滨
4.
哈尔滨

摘要

犬Ⅰ型腺病毒(CAV-1)弱毒用限制性内切酶EcoR Ⅰ,BamH Ⅰ,Pst Ⅰ,Sph Ⅰ和Hind Ⅲ消化分析后其图谱与强毒株相比没有差异。将弱毒DNA用Pst Ⅰ完全消化后以鸟枪法克隆到载体质粒pBluescrip'SK中,经用光生物素标记的CAV-1 DNA杂交筛选以及Pst Ⅰ分析重组质粒证明已将分子量为5.5,3.5,2.85,1.2,0.32和0.28Kb的CAV-1DNA片段克隆到质粒中。克隆到的这些片段将可考虑进一步研究作为探针检测犬及狐狸等野生动物的腺病毒感染。
- 犬1型腺病毒
- , DNA酶切分析
- , 分子克隆

Restriction Enzyme Analysis and Molecular Cloning of CAV-1 DNA

1.
The National Veterinary biotechnological Laboratory
2.
Harbin Vet. Res. Inst. Harbin 150001The Military Veterinary Research Institute
3.
Vet. College of PLA
4.
Changchun 130012

Abstract

The virulent and modified canine adenovirus type 1 (CAV-1) DNAs extracted from canine kidney cells (MDCK) 36 hrs postinfection were both analysed with restriction enzymes EcoR Ⅰ, BamH Ⅰ, Pst Ⅰ, Nsi Ⅰ, Sph Ⅰ and Hind Ⅲ. and no difference was found from the electrophoresis pattern between the two viruses. The modified CAV-1 DNA was digested completely with Pst Ⅰ and cloned into, plasmid pBluescript SK taking shotgun procedure. Through hybridization selection of photobiotin labeled CAV-1 DNA and Pst Ⅰ digestio...
- CAV-1 DNA fingerprinting Molecular cloning

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Restriction Enzyme Analysis and Molecular Cloning of CAV-1 DNA

1. The National Veterinary biotechnological Laboratory
2. Harbin Vet. Res. Inst. Harbin 150001The Military Veterinary Research Institute
3. Vet. College of PLA
4. Changchun 130012

Abstract: The virulent and modified canine adenovirus type 1 (CAV-1) DNAs extracted from canine kidney cells (MDCK) 36 hrs postinfection were both analysed with restriction enzymes EcoR Ⅰ, BamH Ⅰ, Pst Ⅰ, Nsi Ⅰ, Sph Ⅰ and Hind Ⅲ. and no difference was found from the electrophoresis pattern between the two viruses. The modified CAV-1 DNA was digested completely with Pst Ⅰ and cloned into, plasmid pBluescript SK taking shotgun procedure. Through hybridization selection of photobiotin labeled CAV-1 DNA and Pst Ⅰ digestio...