The Application of Rapid HEPES-plaques-forming Test(RHPFT)to Rabies Virus Titration

SHAO Yi-Bin; GU  Qin; ZENG Rong-Fang

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February 1994

SHAO Yi-Bin, GU Qin and ZENG Rong-Fang. The Application of Rapid HEPES-plaques-forming Test(RHPFT)to Rabies Virus Titration[J]. Virologica Sinica, 1994, 9(1).

Citation: SHAO Yi-Bin, GU Qin, ZENG Rong-Fang. The Application of Rapid HEPES-plaques-forming Test(RHPFT)to Rabies Virus Titration .VIROLOGICA SINICA, 1994, 9(1) : 70.

应用快速HEPES蚀斑法检测狂犬病毒

1.
卫生部上海生物制品研究所

摘要

应用快速ＨＥＰＥＳ蚀斑法检测狂犬病毒毒力滴定，狂犬病毒蚀斑法作为滴定活性病毒效价常用的比较精确的方法早被广泛应用。国内外学者已经建立并应用狂...
- HEPFS蚀斑法 MC蚀斑法小鼠脑内毒力滴定狂犬病毒

The Application of Rapid HEPES-plaques-forming Test(RHPFT)to Rabies Virus Titration

1.
Institute of Shanghai Biological Products

Abstract

According to the phenomenon that HEPES could enhance CPE in rabies virus infected cell and accelerate the plaques formation,the virulence of rabies virus(RFD strain)was detected with 3 differentmethods includig RHPFT,MC-plaques test and mollse i.c,test.The results showed that the sensibility of 3 detecting methods was similar but RHPFT was more rapid and simple than the latter two,Theresult of RHPFT methed could be obtained earlier about 6-7 days than MC-plaques test which everused as a routine assay in rabies virus titration.RIlPFT could also be used for rabies neutralizing anti-body assay.
- HEPES-plaques-forming test MC-plaques-forming test Mouse i.c.test Rabies virus

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

The Application of Rapid HEPES-plaques-forming Test(RHPFT)to Rabies Virus Titration

1. Institute of Shanghai Biological Products

Abstract: According to the phenomenon that HEPES could enhance CPE in rabies virus infected cell and accelerate the plaques formation,the virulence of rabies virus(RFD strain)was detected with 3 differentmethods includig RHPFT,MC-plaques test and mollse i.c,test.The results showed that the sensibility of 3 detecting methods was similar but RHPFT was more rapid and simple than the latter two,Theresult of RHPFT methed could be obtained earlier about 6-7 days than MC-plaques test which everused as a routine assay in rabies virus titration.RIlPFT could also be used for rabies neutralizing anti-body assay.