Detection and Significance of HCV RNA NSs Gene

GUO Lin-Sheng; TANG Zhen-E; HAO Lian-Jie

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April 1994

GUO Lin-Sheng, TANG Zhen-E and HAO Lian-Jie. Detection and Significance of HCV RNA NSs Gene[J]. Virologica Sinica, 1994, 9(2): 119-124.

Citation: GUO Lin-Sheng, TANG Zhen-E, HAO Lian-Jie. Detection and Significance of HCV RNA NSs Gene .VIROLOGICA SINICA, 1994, 9(2) : 119-124.

血清HCV RNA NS基因的检测及其意义

1.
同济医科大学同济医院临床免疫研究室，武汉430030

摘要

利用源自ＮＳ５和５－ＵＴＲ引物进行丙型肝炎病毒（ＨＣＶ）ＲＮＡ基因片段的逆转录－套式多聚酶链反应（ＲＴ－ｎｅａｔｅｄＰＣＲ）检测。对３５例随机选择的抗ＨＣＶＡｂ（＋）血清的检测结果表明，两种引物检测结果的敏感性无显著性差别。利用ＮＳ５区引物扩增的片段可与５种不同型别的ＨＣＶＲＮＡ亚基因探针很好地结合和显色，说明ＮＳ５基因可以利用于ＨＣＶＲＮＡ的基因分型。对１３１例抗ＨＣＶＡｂ（＋）血清进行了ＮＳ５基因检测，检出率为５２．６８％（６９／１３１），其小，除ＨＣＣ和ＣＲＦ组检出率分别为１６．６７％和３４．６８％，其余各组均在５０～８０％之间。献血组、献浆组和免疫球蛋白中ＨＣＶＲＮＡ的检出率分别为６６．６１％，５４．４５％和５７．１４％，说明应用未经筛查的血液及血制品小有很高的感染危险性，２２例抗ＨＣＶＡｂ（－）ＮＡＮＢＨ患者中有８例第２次ＰＣＲ后确证为ＨＣＶＲＮＡ（＋），表明１／３与低水平的ＨＣＶ感染有关。
- HCVRNA
- , NS基因
- , 抗丙型肝炎病毒抗体
- , 逆转录
- , 套式多聚酶链反应

Detection and Significance of HCV RNA NSs Gene

1.
Chitial Immunalogy Research unil,Tongji Hospital Wuhan 430030

Abstract

rimer selection is important for the detection of HCV RNA with RT-nested PCR method Com.pered to primers from 5'-UTR gene,the primere from NS5 gene had also high sensitivity and specifityin the detection of HCV RNA.Sixty-nine of 131 cases of anti-HCV Ab positive and 8 of 22 cases ofanti-HCV Ab nesative sera were found to be NS_5 gene positive,the positive rate were 52. 68% and36.36%,resnytively.All anti-HCV Ab positive samplea were divided into several sroups,according totheir clinical beckground.Prevalence of NS_5 gene in HCC and CRF groupe were 16.67% and 34.38%,respectively,lowertian that of other groupe (50%-80%),Interestinsly,the patientswith active liver diseiises(such as HCC,CAH,LC,SH) were peitive in the lst PCR,then the patientswith porristent hepotitis and subclinical infection were peitive in the 2nd PCR.The high prevalence ofNS5 gene in groupe of BD,PD and IG warned us of screening BD strictly and using blood-preducts carefully.
- HCV RNA
- , NS gene
- , RT nested PCR
- , Anti HCV Ab

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Detection and Significance of HCV RNA NSs Gene

1. Chitial Immunalogy Research unil,Tongji Hospital Wuhan 430030

Abstract: rimer selection is important for the detection of HCV RNA with RT-nested PCR method Com.pered to primers from 5'-UTR gene,the primere from NS5 gene had also high sensitivity and specifityin the detection of HCV RNA.Sixty-nine of 131 cases of anti-HCV Ab positive and 8 of 22 cases ofanti-HCV Ab nesative sera were found to be NS_5 gene positive,the positive rate were 52. 68% and36.36%,resnytively.All anti-HCV Ab positive samplea were divided into several sroups,according totheir clinical beckground.Prevalence of NS_5 gene in HCC and CRF groupe were 16.67% and 34.38%,respectively,lowertian that of other groupe (50%-80%),Interestinsly,the patientswith active liver diseiises(such as HCC,CAH,LC,SH) were peitive in the lst PCR,then the patientswith porristent hepotitis and subclinical infection were peitive in the 2nd PCR.The high prevalence ofNS5 gene in groupe of BD,PD and IG warned us of screening BD strictly and using blood-preducts carefully.