WANG Xiao-Rong, LIU Ming-Fu, LIU Run-Zhong, LAN Ping-Zhang and ZHANG You-Qing. Toxicity Test and Analysis of Genomic DNA of Spodoptera litura Nuclear Polyhedrosis Virus[J]. Virologica Sinica, 1994, 9(4).
Citation:
WANG Xiao-Rong, LIU Ming-Fu, LIU Run-Zhong, LAN Ping-Zhang, ZHANG You-Qing.
Toxicity Test and Analysis of Genomic DNA of Spodoptera litura Nuclear Polyhedrosis Virus .VIROLOGICA SINICA, 1994, 9(4)
: 378.
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摘要
一株斜纹夜蛾核型多角体病毒毒力及基因组酶切的研究王晓容,刘明富,刘润忠,兰萍章,张友清(中国科学院武汉病毒研究所,武汉430071)关键词斜纹夜蛾,核多角体病毒,毒力测定,限制酶分析斜纹夜蛾(Spodopleralitura)是重要的农业害虫之一。关...
Toxicity Test and Analysis of Genomic DNA of Spodoptera litura Nuclear Polyhedrosis Virus
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Abstract
The toxicity of a strain of Spodoptera tilura nuclear polyhedrosis virus(SINPV)was tested.The 4th instar larvae of Spodoptera titura were used to carryout the bioassays.LC5e=2.014×104PIBs/eachlarva.The genomic DNA was digested with three restriction endonucleases BamHI,EcoRI and PstI,and 17,22 and 12 fragments were produced,respectively,The total genome size is 113kb.
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