Characterization of Epstein—Barr Virus Gene Detected
in Nasopharyngeal Carcinoma

JIANG Zhi-Long; LU Jian; CHEN Shi-Shu

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February 1995

JIANG Zhi-Long, LU Jian and CHEN Shi-Shu. Characterization of Epstein—Barr Virus Gene Detectedin Nasopharyngeal Carcinoma[J]. Virologica Sinica, 1995, 10(1).

Citation: JIANG Zhi-Long, LU Jian, CHEN Shi-Shu. Characterization of Epstein—Barr Virus Gene Detected in Nasopharyngeal Carcinoma .VIROLOGICA SINICA, 1995, 10(1) : 25.

鼻咽癌中Epstein—Barr病毒基因结构特点

1.
上海第二医科大学生物化学教研室分子生物学实验室

摘要

鼻咽癌中感染的ＥＢＶ以潜伏态存在，只有少数基因得到表达。我们从分子生物学水平研究其潜伏机制。已知ＢＺＬＦｌ基因和ＢａｍＨＩＥＺ片段内部分基因对ＥＢＶ的复制激活起关键作用。我们用体外基因扩增（ＰＣＲ）法扩增ＢＺＬＦｌ基因外显子－１序列，并用序列分析法研究了该基因结构特点。此外，检测了ＢａｍＨＩＥＺ片段内缺失，结果发现２１例鼻咽癌（ＮＰＣ）组织中，ｌｌ例有ＢａｍＨＩＥＺ片段内缺失，长２．９９ｋｂ，与潜伏态存在的ＲａｊｉＥＢＶ类似。提示鼻咽癌小的ＥＢＶ以潜伏态存在，与ＮＰＣ发病有关。
- 鼻咽癌（NPC） EBV 多聚酶链反应 DNA序列分析

Characterization of Epstein—Barr Virus Gene Detected in Nasopharyngeal Carcinoma

1.
Shanghai Second Medical University

Abstract

We analysed the BamHl EZ fragment of Epstein—Barr Virus in 21 cases of tissues from nas opha．ry'ngeal c~rcinoma(NPC)by polymerase chain reaction(PCR)and,r~quence analysis．The 2．99kb deletions within lhmHl EZ fragment were found in 1 1 sfm1DIes，the deletions wei'~similar to those 0f Raji EBV which is latent in Burkitt s lymphoma．The BZLF1 gene of EBV locates within Bam HI EZ fragment an d has an impor~nt role in reproduc~ve cycle of EBV．Two point m utatioas were also found in the first exon of BZLFt gcne from a sam ple analyzed ，com pared to the standard B9S．8 EBV． The m uta tions above may affect the latency~disrupting function of Epstein Barr Virus
- Nasopharynse,al carcinoma(NPC)

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Characterization of Epstein—Barr Virus Gene Detected in Nasopharyngeal Carcinoma

1. Shanghai Second Medical University

Abstract: We analysed the BamHl EZ fragment of Epstein—Barr Virus in 21 cases of tissues from nas opha．ry'ngeal c~rcinoma(NPC)by polymerase chain reaction(PCR)and,r~quence analysis．The 2．99kb deletions within lhmHl EZ fragment were found in 1 1 sfm1DIes，the deletions wei'~similar to those 0f Raji EBV which is latent in Burkitt s lymphoma．The BZLF1 gene of EBV locates within Bam HI EZ fragment an d has an impor~nt role in reproduc~ve cycle of EBV．Two point m utatioas were also found in the first exon of BZLFt gcne from a sam ple analyzed ，com pared to the standard B9S．8 EBV． The m uta tions above may affect the latency~disrupting function of Epstein Barr Virus