The Screen of Inhibitors of Grass Carp Hemorrhage Virus
from a Phage Displayed Random Peptide Library

WANG Bing; KE Li-Hua; JIANG Hong; TIAN Po; LI Chuan-Zhao

PDF
Cite
Share

facebook

twitter

google

linkedin

August 1998

WANG Bing, KE Li-Hua, JIANG Hong, TIAN Po and LI Chuan-Zhao. The Screen of Inhibitors of Grass Carp Hemorrhage Virusfrom a Phage Displayed Random Peptide Library[J]. Virologica Sinica, 1998, 13(4).

Citation: WANG Bing, KE Li-Hua, JIANG Hong, TIAN Po, LI Chuan-Zhao. The Screen of Inhibitors of Grass Carp Hemorrhage Virus from a Phage Displayed Random Peptide Library .VIROLOGICA SINICA, 1998, 13(4) : 351.

从随机噬菌体肽库中筛选抗草鱼出血病病毒多肽的研究

1.
中国科学院武汉病毒研究所中国科学院微生物研究所

摘要

从随机噬菌体九肽表位库中筛选出抑制草鱼出血病病毒（ＧｒａｓＣａｒｐＨｅｍｏｒｈａｇｅＶｉｒｕｓ，ＧＣＨＶ８７３）感染的多肽分子。采用完整、生物素化的草鱼出血病病毒颗粒作为受体，与以融合蛋白形式在丝状噬菌体ｆＵＳＥ５的外壳蛋白Ⅲ的Ｎ端表达的随机九肽库作用。经三轮体外亲和筛选（Ｂｉｏｐａｎｎｉｎｇ）及ＥＬＩＳＡ法检测后，从肽库中筛选出１６个与病毒高亲和力结合的噬菌体克隆，其中六个阳性克隆能有效地抑制病毒在ＣＩＫ细胞中的复制，并使病毒的半数组织培养感染剂量（ＴＣＩＤ５０）下降五个数量级。表明噬菌体肽库技术完全能够应用于抗病毒研究，为研制抗病毒短肽制剂打基础。
- 随机噬菌体九肽库体外亲和筛选草鱼出血病毒（GCHV）873 抗病毒多肽

The Screen of Inhibitors of Grass Carp Hemorrhage Virus from a Phage Displayed Random Peptide Library

1.
Wuhan Institute of Virology，Chinese Academy of Sciences．Wuhan 430071Institute of Microbiology．Chinese Academy of Sciences Beijing 100080

Abstract

Screening inhibitors of GCHV 873 (Grass Carp Hemorrhage Virus)to inhibit its replication in CIK cells in vitro ,the biotinylated intact virion of GCHV was used to react on a random sequenced peptide library of 9 amino acids fused on protein Ⅲ of filamentous phage fUSE5 .After 3 rounds of affinity selection,immuno screening and ELISA,16 positive clones were selected using CsCl gradient purified virus particles, 6 of them inhibited the replication of GCHV in CIK cells in vitro and decreased the TCID 50 of GCHV from 10 -10 to 10 -5 .These findings open possibility of identifying virus proteins or epitopes as targets for antiviral agents.
- Random peptide library Biopanning in vitro Grass Carp Hemorrhage Virus Antiviral peptides

References
Proportional views

PDF

Article Metrics

Article views(3742) PDF downloads(855) Cited by(0)

Proportional views

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

The Screen of Inhibitors of Grass Carp Hemorrhage Virus from a Phage Displayed Random Peptide Library

1. Wuhan Institute of Virology，Chinese Academy of Sciences．Wuhan 430071Institute of Microbiology．Chinese Academy of Sciences Beijing 100080

Abstract: Screening inhibitors of GCHV 873 (Grass Carp Hemorrhage Virus)to inhibit its replication in CIK cells in vitro ,the biotinylated intact virion of GCHV was used to react on a random sequenced peptide library of 9 amino acids fused on protein Ⅲ of filamentous phage fUSE5 .After 3 rounds of affinity selection,immuno screening and ELISA,16 positive clones were selected using CsCl gradient purified virus particles, 6 of them inhibited the replication of GCHV in CIK cells in vitro and decreased the TCID 50 of GCHV from 10 -10 to 10 -5 .These findings open possibility of identifying virus proteins or epitopes as targets for antiviral agents.