将草鱼出血病病毒（ＧＣＨＶ８７３）颗粒与随机噬菌体九肽库在体外作用，三轮筛选后，从３００个转化的单菌落中获得１６个与病毒高亲和力的噬菌体克隆。接着经过抗病毒试验获得６个能强烈抑制病毒复制的阳性噬菌体克隆，能使病毒ＴＣＩＤ５０下降５个数量级。通过对阳性噬菌体克隆随机插入区域的核苷酸序列分析，推导出多肽的氨基酸序列。发现６个能强烈抑制病毒复制的阳性克隆中多肽的氨基酸序列完全一致（ＮＨ２ＬｅｕＴｒｐＶａｌＧｌｙＧｌｙＧｌｙＡｒｇＡｓｎＡｌａ），该结果提示多肽的抗病毒能力与多肽特异性氨基酸组成及结构有关。因此，抑制ＧＣＨＶ８７３复制特异性多肽的氨基酸序列的确定及结构分析，不仅为人工合成抗草鱼出血病病毒的多肽奠定了基础，同时也为抗病毒多肽制剂的研制提供了依据。

Through the reaction between GCHV 873 (Grass Carp Hemorrhage Virus) particles and random peptide library displayed on phage in vitro, after 3 rounds of affinity selection 16 phage clones from 300 clones have been got, which have high affinity with GCHV. Six of sixteen clones could inhibit the replication of GCHV in CIK in vitro and decrease the TCID 50 to half of index. The DNA sequence of phage clones and deduced sequence of amino acid of the inserted foreign fragments were analysed. The results show that the sequences of six clones are identical. The deduced sequence of amino acid is NH2 Leu Trp Val Gly Gly Gly Arg Asn Ala. It indicates that the ability of anti GCHV is certainly related to specific structural information and amino acid sequence of peptides. The determination of antiviral peptide offers a fundamental practical advance in the synthesis of the antiviral peptides and provides a way of generating noval antiviral agents.