Detection of HAV contamination in shellfish from Lianyungang sea water by 
antibody capture- PCR

ZHAO Wen-Ban; LIN Hong-Yu; LI Jia-Fu; WANG Li-Xin; GONG Hai-Ping; FANG Zhao-Yin; WANG Qiu-Gong

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August 1998

ZHAO Wen-Ban, LIN Hong-Yu, LI Jia-Fu, WANG Li-Xin, GONG Hai-Ping, FANG Zhao-Yin and WANG Qiu-Gong. Detection of HAV contamination in shellfish from Lianyungang sea water by antibody capture- PCR[J]. Virologica Sinica, 1998, 13(4).

Citation: ZHAO Wen-Ban, LIN Hong-Yu, LI Jia-Fu, WANG Li-Xin, GONG Hai-Ping, FANG Zhao-Yin, WANG Qiu-Gong. Detection of HAV contamination in shellfish from Lianyungang sea water by antibody capture- PCR .VIROLOGICA SINICA, 1998, 13(4) : 372.

AC-PCR法检测连云港海域贝类HAV

1.
连云港市卫生防疫站中国预防医学科学院病毒学研究所

摘要

为了解连云港海域贝类甲型肝炎病毒（ＨＡＶ）污染状况，证实其在本地区甲肝传播中的媒介地位，我们应用抗体捕捉聚合酶链反应（ＡＣ／ＰＣＲ）检测市售贝类的ＨＡＶ，结果报告如下：材料和方法１贝类样品于１９９６年春、秋二季采集新浦区、连云区和赣榆县等地水产品市场...
- 贝类甲型肝炎病毒聚合酶链反应

Detection of HAV contamination in shellfish from Lianyungang sea water by antibody capture- PCR

1.
Lianyungang City Sanitary and Anti-epidemic Station．Lianyungang 222003

Abstract

A method of antibody capture polymerase chain reaction (AC PCR) is developed for the detection of human hepatitis A virus (HAV) in shellfish ( Scapharca subcrenata, Buditapes philippinarum, Macoma incongrua, Sinonovacula constricta, Chlamys farrer ). Initial virus extraction is based on a modified elution precipitation technique. Eleven out of 31 shellfish samples from Lianyungang (in Jiangsu Province) sea water are HAV positive. This experiment proves that the consumption of shellfish growing in contamination waters is associated with the risk of transmission of HAV.
- Shellifish HAV PCR

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Detection of HAV contamination in shellfish from Lianyungang sea water by antibody capture- PCR

1. Lianyungang City Sanitary and Anti-epidemic Station．Lianyungang 222003

Abstract: A method of antibody capture polymerase chain reaction (AC PCR) is developed for the detection of human hepatitis A virus (HAV) in shellfish ( Scapharca subcrenata, Buditapes philippinarum, Macoma incongrua, Sinonovacula constricta, Chlamys farrer ). Initial virus extraction is based on a modified elution precipitation technique. Eleven out of 31 shellfish samples from Lianyungang (in Jiangsu Province) sea water are HAV positive. This experiment proves that the consumption of shellfish growing in contamination waters is associated with the risk of transmission of HAV.