New Nucleotide Sequences of the 5 Non-coding Region of Hepatitis C Virus Isolated from China

CHEN Yuan-Ding; LIU Ming-Yang; LI Jia-Qi; DIAO Wei; BANG Mei; ZHOU Ceng-Quan; TU Wen-Lin; LI Hui-Qiong-

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April 2000

CHEN Yuan-Ding, LIU Ming-Yang, LI Jia-Qi, DIAO Wei, BANG Mei, ZHOU Ceng-Quan, TU Wen-Lin and LI Hui-Qiong-. New Nucleotide Sequences of the 5 Non-coding Region of Hepatitis C Virus Isolated from China[J]. Virologica Sinica, 2000, 15(2): 136-142.

Citation: CHEN Yuan-Ding, LIU Ming-Yang, LI Jia-Qi, DIAO Wei, BANG Mei, ZHOU Ceng-Quan, TU Wen-Lin, LI Hui-Qiong-. New Nucleotide Sequences of the 5 Non-coding Region of Hepatitis C Virus Isolated from China .VIROLOGICA SINICA, 2000, 15(2) : 136-142.

中国丙型肝炎患者中发现新的丙型肝炎病毒核苷酸序列

1.
中国医学科学院中国协和医科大学医学生物学研究所,昆明650107

摘要

应用RTPCR和DNA克隆重组技术,从中国云南地区丙型肝炎患者血清中克隆到3个丙型肝炎病毒(HCV)5端非编码区(5NCR)核苷酸序列。这3个序列分别来自3个不同的丙型肝炎患者。与国内外已发表的HCV原型株同源序列比较研究发现,这3个HCV5NCR序列中分别存在部分核苷酸序列的缺失或出现插入重复。在YS22515NCR序列-155～-174位之间,有18个核苷酸序列缺失;在YS2034和YS24215NCR序列-55～-56位之间,分别有28个(YS2034)和40个(YS2421)核苷酸序列插入。这些插入序列(28个核苷酸)在其相邻部位已有存在,即为重复序列。YS2421插入序列中有11个核苷酸出现两次重复。其它部位的核苷酸变异也同已知基因型的相应核苷酸变异有很大差异。结果表明,这3个序列是迄今为止国内外都未曾发现过的新的HCV核苷酸序列
- 中国丙型肝炎患者
- , 丙型肝炎病毒5
- , 端非编码区
- , 核苷酸序列
- , 缺失
- , 插入
- , HCV核苷酸序列

New Nucleotide Sequences of the 5 Non-coding Region of Hepatitis C Virus Isolated from China

1.
Institute of Medlical Biology,Chinese Academy of Medical

Abstract

From the sera of 3 Chinese patients with hepatitis C, 3 new nucleotide sequences of the 5 non coding region (5NCR) of hepatitis C virus (HCV) were found using reverse transcription polymerase chain reaction (RT PCR) and DNA recombinant and sequencing techniques. Comparing with previously published HCV corresponding sequences, these new sequences have some consecutive nucleotides deleted or inserted. Sequence YS225 1 has 18 nucleotides deletion between the original position 174～-155 compared with the corresponding sequence of the prototype, HCV 1 (gene 1a); while sequence YS203 4 and sequence YS242 1 respectively contain extra 28 nucleotides and 40 nucleotides insertion between the original position -56～-55. These nucleotides are the repeating sequences existing in the forward sense (original position -83～-56). In sequence YS242 1, there is a sequence containing 11 nuclotides (corresponding to the original position -76～-56) repeated twice. In addition, variations of the nucleotides among these sequen.
- Chinese patients with hepatitis C
- , HCV 5 NCR
- , Nudeotide sequen ce
- , Deletion
- , Insertion
- , HCV nucleotide sequen ces

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

New Nucleotide Sequences of the 5 Non-coding Region of Hepatitis C Virus Isolated from China

1. Institute of Medlical Biology,Chinese Academy of Medical

Abstract: From the sera of 3 Chinese patients with hepatitis C, 3 new nucleotide sequences of the 5 non coding region (5NCR) of hepatitis C virus (HCV) were found using reverse transcription polymerase chain reaction (RT PCR) and DNA recombinant and sequencing techniques. Comparing with previously published HCV corresponding sequences, these new sequences have some consecutive nucleotides deleted or inserted. Sequence YS225 1 has 18 nucleotides deletion between the original position 174～-155 compared with the corresponding sequence of the prototype, HCV 1 (gene 1a); while sequence YS203 4 and sequence YS242 1 respectively contain extra 28 nucleotides and 40 nucleotides insertion between the original position -56～-55. These nucleotides are the repeating sequences existing in the forward sense (original position -83～-56). In sequence YS242 1, there is a sequence containing 11 nuclotides (corresponding to the original position -76～-56) repeated twice. In addition, variations of the nucleotides among these sequen.