Genotyping and Sequencing analysis of 15 strains of TT Virus from Chinese isolates

WEN Chu-Juan; HUANG Tian-Hua; CHENG Qun; GU Zhen-Mei; HOU Jin-Lin

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June 2000

WEN Chu-Juan, HUANG Tian-Hua, CHENG Qun, GU Zhen-Mei and HOU Jin-Lin. Genotyping and Sequencing analysis of 15 strains of TT Virus from Chinese isolates[J]. Virologica Sinica, 2000, 15(3): 214-219.

Citation: WEN Chu-Juan, HUANG Tian-Hua, CHENG Qun, GU Zhen-Mei, HOU Jin-Lin. Genotyping and Sequencing analysis of 15 strains of TT Virus from Chinese isolates .VIROLOGICA SINICA, 2000, 15(3) : 214-219.

15株中国TT病毒基因型鉴定和部分序列分析

1.
广州南方医院医学基因技术中心，广洲 510515

摘要

TT病毒 (TTV)为一种新鉴定的输血后肝炎相关的病毒。用聚合酶链式反应 (PCR)扩增TTVDNA序列核苷酸 1915到 2 185之间的片段 ,并将该片段克隆入 pGEM TEasy载体。重组克隆经酶切鉴定后 ,用全自动测序仪测序 ,发现本组病例中 15株TTV毒株间的同源性在 6 6 .8%到99.3%之间 ,与TTV日本株比较同源性在 6 6 .1%到 97.4 %之间 ,分析结果显示在我国不仅有国外已报告的不同基因亚型毒株存在 ,而且 ,分离到的 3株TTV毒株与日本株G1组和G2组的异源性分别达到 13.3% - 33.2 % ,可能为新的TTV基因亚型。
- 献血员
- , 肝炎
- , TTV
- , 基因型

Genotyping and Sequencing analysis of 15 strains of TT Virus from Chinese isolates

1.
Genetic Center,Nanfang Hospitat，Guangzhou

Abstract

Recently, a novel virus named TT Virus has been identified in serum from patients with elevated ALT levels and others. TTV was detected by PCR using a set of primers from conserved region in the TTV genome. cloning and sequencing of the amplifications in 15 cases of Chinese blood donors and dialysis patients were carrited out. Sequence comparison within 271 base pairs in the region between positions 1915 and 2185 showed homology rates varying from 66.8% to 99.3% among 15 Chinese isolates, and from 66.1% to 97.4% between Chinese strains and TTV isolates from Japanese isolates sequenced. This result suggests that a new sub genotype existes in Chinese TTV isolates.
- Blood donorI
- , Hepa titis
- , TTv
- , Geno typa

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Genotyping and Sequencing analysis of 15 strains of TT Virus from Chinese isolates

1. Genetic Center,Nanfang Hospitat，Guangzhou

Abstract: Recently, a novel virus named TT Virus has been identified in serum from patients with elevated ALT levels and others. TTV was detected by PCR using a set of primers from conserved region in the TTV genome. cloning and sequencing of the amplifications in 15 cases of Chinese blood donors and dialysis patients were carrited out. Sequence comparison within 271 base pairs in the region between positions 1915 and 2185 showed homology rates varying from 66.8% to 99.3% among 15 Chinese isolates, and from 66.1% to 97.4% between Chinese strains and TTV isolates from Japanese isolates sequenced. This result suggests that a new sub genotype existes in Chinese TTV isolates.