Studieson Association of the Replication of AcMNPV with the Nuclear Matrix

LIU Xiao-Qiong; TU Ze-Hua; WANG Jia-Kun; TAO Han-Chao; GUO Hua-Shan; CHEN Qu-Hou-

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August 2000

LIU Xiao-Qiong, TU Ze-Hua, WANG Jia-Kun, TAO Han-Chao, GUO Hua-Shan and CHEN Qu-Hou-. Studieson Association of the Replication of AcMNPV with the Nuclear Matrix[J]. Virologica Sinica, 2000, 15(4): 350-356.

Citation: LIU Xiao-Qiong, TU Ze-Hua, WANG Jia-Kun, TAO Han-Chao, GUO Hua-Shan, CHEN Qu-Hou-. Studieson Association of the Replication of AcMNPV with the Nuclear Matrix .VIROLOGICA SINICA, 2000, 15(4) : 350-356.

苜蓿丫纹夜蛾核多角体病毒复制与宿主细胞核骨架关系初探

1.
华中师范大学昆虫学研究所，武汉 4300

摘要

用选择性抽提方法和整装细胞电镜技术观察苜蓿丫纹夜蛾多粒包埋核型多角体病毒 (AcM NPV)感染的Tn5B1细胞的核骨架结构体系 ,发现感染早期病毒的复制过程未对宿主细胞核骨架的形态结构产生明显影响 ,而感染晚期多角体的装配在核骨架网络中进行。以ie 1基因和多角体蛋白基因为探针 ,点杂交分析基因转录活性与宿主细胞核骨架的关系 ,结果表明在病毒感染早期 ,无论是正具转录活性的ie 1基因还是不具转录活性的多角体蛋白基因都可紧密结合在宿主细胞的核骨架上。
- 核骨架
- , AcMNPV 复制
- , 多角体装配
- , 基因转录活性

Studieson Association of the Replication of AcMNPV with the Nuclear Matrix

1.
Institute of Entomology ，Central China Normal Unitersity,Wuhan 430079．China

Abstract

The structure of nuclear matrix in Tn5B1 cell infected with AcMNPV was observed by means of sequential cell fractionation and whole mount technique of electron microscopy. Whereas early infection of AcMNPV resulted in no dramatic change in the nuclear matrix morphologically, polyhedra formed in the network of the nuclear matrix in the late stage of infection. Results of Dot boltting showed that both ie 1 gene and polyhedrin gene tightly binded on the nuclear matrix in early stage of infection.
- Nuclear matrix AcMNPV
- , Replication
- , Polyhedra assembly
- , Transcriptional activit

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Studieson Association of the Replication of AcMNPV with the Nuclear Matrix

1. Institute of Entomology ，Central China Normal Unitersity,Wuhan 430079．China

Abstract: The structure of nuclear matrix in Tn5B1 cell infected with AcMNPV was observed by means of sequential cell fractionation and whole mount technique of electron microscopy. Whereas early infection of AcMNPV resulted in no dramatic change in the nuclear matrix morphologically, polyhedra formed in the network of the nuclear matrix in the late stage of infection. Results of Dot boltting showed that both ie 1 gene and polyhedrin gene tightly binded on the nuclear matrix in early stage of infection.