Cloning of Grapevine Leafroll Associated Virus 3 Coat Protein Gene

DIAO Dong-Lan; SHU Shui-Fang; HUANG Wen-Qing; ZHANG Man-Liang

PDF
Cite
Share

facebook

twitter

google

linkedin

August 2000

DIAO Dong-Lan, SHU Shui-Fang, HUANG Wen-Qing and ZHANG Man-Liang. Cloning of Grapevine Leafroll Associated Virus 3 Coat Protein Gene[J]. Virologica Sinica, 2000, 15(4): 357-360.

Citation: DIAO Dong-Lan, SHU Shui-Fang, HUANG Wen-Qing, ZHANG Man-Liang. Cloning of Grapevine Leafroll Associated Virus 3 Coat Protein Gene .VIROLOGICA SINICA, 2000, 15(4) : 357-360.

葡萄卷叶伴随病毒Ⅲ外壳蛋白基因的克隆

1.
徐卅l市农科所，江苏徐州 221121

摘要

根据美国报道的葡萄卷叶伴随病毒Ⅲ (GLRaV 3)CP基因序列 ,设计并合成一对引物 ,用IC RT PCR对GLRaV 3进行检测 ,琼脂糖凝胶电泳表明其大小与预计相符。将其CP基因克隆到pGEM 3zf( +)中 ,经双酶切和序列分析表明所克隆的是GLRaV 3CP基因 ,它与美国分离物相应序列同源性为 94 .1%。
- 葡萄卷叶病毒
- , GLRaV-3
- , IC-RT-PCR
- , CP
- , 克隆

Cloning of Grapevine Leafroll Associated Virus 3 Coat Protein Gene

1.
Xuzhou AgriculturatResearch Institute，Xuzhou．Jiangsu，221121 China

Abstract

A pair of primers were designed and synthesized based on the nucleotide sequence of coat protein gene of GLRaV 3 from America. The expected size of CP gene of GLRaV 3 was amplified by IC (Immuno capture) RT PCR method. The CP gene of GLRaV 3 was cloned into pGEM 3zf(+) vector and sequenced. The nucleotide sequence was compared with American isolate. Result showed that their homology was 94.1%.
- Grapevine leafroll virus
- , GLRaV 3
- , IC RT PCR
- , CP
- , Cl

References
Proportional views

PDF

Article Metrics

Article views(3713) PDF downloads(772) Cited by(0)

Proportional views

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Cloning of Grapevine Leafroll Associated Virus 3 Coat Protein Gene

1. Xuzhou AgriculturatResearch Institute，Xuzhou．Jiangsu，221121 China

Abstract: A pair of primers were designed and synthesized based on the nucleotide sequence of coat protein gene of GLRaV 3 from America. The expected size of CP gene of GLRaV 3 was amplified by IC (Immuno capture) RT PCR method. The CP gene of GLRaV 3 was cloned into pGEM 3zf(+) vector and sequenced. The nucleotide sequence was compared with American isolate. Result showed that their homology was 94.1%.