Com parative Analysis of Serological and M olecular M ethods for the detection of Rice Grassy Stunt Virus

ZHANG Chun-Mei; LIN Ai-Yang; XIE Lian-Hui

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August 2000

ZHANG Chun-Mei, LIN Ai-Yang and XIE Lian-Hui. Com parative Analysis of Serological and M olecular M ethods for the detection of Rice Grassy Stunt Virus[J]. Virologica Sinica, 2000, 15(4): 361-366.

Citation: ZHANG Chun-Mei, LIN Ai-Yang, XIE Lian-Hui. Com parative Analysis of Serological and M olecular M ethods for the detection of Rice Grassy Stunt Virus .VIROLOGICA SINICA, 2000, 15(4) : 361-366.

水稻草矮病毒血清学和分子检测方法的比较(英文)

1.
福建省植物病毒学重点实验室，福建农业大学植物病毒研究所，福州350002

摘要

将间接ELISA、非放射性分子杂交和RT PCR三种方法应用于水稻草矮病毒 (RGSV)的检测。结果表明 ,利用自制的融合蛋白GST NC的抗血清检测RGSV的灵敏度为 1mg鲜重的病株叶片或 84ng提纯病毒 ,利用地高辛 (DIG)标记的DNA探针NC的点杂交方法检测RGSV的灵敏度为 50 g病叶或 6ng病毒 ,而RT PCR的检测灵敏度则为 10 g病叶或 2ng的病毒 ,对上述三种方法的灵敏度和可操作性也进行了比较。
- 水稻草矮病毒
- , 血清学检测
- , 分子检测

Com parative Analysis of Serological and M olecular M ethods for the detection of Rice Grassy Stunt Virus

1.
Fulian Key Laboratory for Plant Virology，Institute of

Abstract

Methods of ELISA, nonradioactive molecular hybridization and RT PCR were applied in the detection of rice grassy stunt virus (RGSV). The detection sensitivity of indirect ELISA using antiserum against fusion protein GST NC was 1 mg of infected leaves or 84 ng of purified virus. The method of dot hybridization using NC, a DIG labelled DNA probe was 50 g diseased leaves, or 6 ng purified preparations. The detection endpoint of RT PCR was 10 g diseased leaves, or 2 ng purified virus preparation. Comparisons of sensitivity and maneuverability were made among these methods.
- Rice grassy stunt virus
- , serological detection
- , molecular detecti

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Com parative Analysis of Serological and M olecular M ethods for the detection of Rice Grassy Stunt Virus

1. Fulian Key Laboratory for Plant Virology，Institute of

Abstract: Methods of ELISA, nonradioactive molecular hybridization and RT PCR were applied in the detection of rice grassy stunt virus (RGSV). The detection sensitivity of indirect ELISA using antiserum against fusion protein GST NC was 1 mg of infected leaves or 84 ng of purified virus. The method of dot hybridization using NC, a DIG labelled DNA probe was 50 g diseased leaves, or 6 ng purified preparations. The detection endpoint of RT PCR was 10 g diseased leaves, or 2 ng purified virus preparation. Comparisons of sensitivity and maneuverability were made among these methods.