Detection of Papaya Ringspot Virus by Imm uno-capture PCR，Nested-PCR and ELISA-PCR Assays

XIAO Huo-Gen; HU Jin-Sheng; FAN Fu-Zhong

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August 2000

XIAO Huo-Gen, HU Jin-Sheng and FAN Fu-Zhong. Detection of Papaya Ringspot Virus by Imm uno-capture PCR，Nested-PCR and ELISA-PCR Assays[J]. Virologica Sinica, 2000, 15(4): 367-372.

Citation: XIAO Huo-Gen, HU Jin-Sheng, FAN Fu-Zhong. Detection of Papaya Ringspot Virus by Imm uno-capture PCR，Nested-PCR and ELISA-PCR Assays .VIROLOGICA SINICA, 2000, 15(4) : 367-372.

番木瓜环斑病毒PCR检测技术研究

1.
华南农业大学植物病毒研究室，广州510642

摘要

建立了检测番木瓜环斑病毒 (PRV)的免疫捕捉 PCR (IC PCR)法、ELISA PCR法和巢式 PCR法 ,它们分别能从 10 - 4、10 - 7和 10 - 14 稀释度的番木瓜叶粗汁液 (所含鲜叶组织的量分别 0 .5ug、0 .5ng和 5 10 - 6ng)中检测出PRV。
- 番木瓜环斑病毒
- , 免疫捕捉-PCR法
- , ELISA-PCR法
- , 巢式-PCR法

Detection of Papaya Ringspot Virus by Imm uno-capture PCR，Nested-PCR and ELISA-PCR Assays

1.
Lab of Plant

Abstract

Sensitive, rapid, and reliable immuno capture PCR (IC PCR), ELISA PCR and Nested PCR assays were developed for the first time to detect PRV. It was detected from dilutions of 10 -4 , 10 -7 and 10 -14 (equivalent to 0.5 ug, 0.5 ng and 510 -8 ng of papaya leaf tissues) by IC PCR, ELISA PCR and Nested PCR, respectively.
- Papaya ringspot virus
- , Reverse transcription PCR
- , Immunocapture PCR
- , Nested PCR

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Detection of Papaya Ringspot Virus by Imm uno-capture PCR，Nested-PCR and ELISA-PCR Assays

1. Lab of Plant

Abstract: Sensitive, rapid, and reliable immuno capture PCR (IC PCR), ELISA PCR and Nested PCR assays were developed for the first time to detect PRV. It was detected from dilutions of 10 -4 , 10 -7 and 10 -14 (equivalent to 0.5 ug, 0.5 ng and 510 -8 ng of papaya leaf tissues) by IC PCR, ELISA PCR and Nested PCR, respectively.