Analysis of HIV-1 Sequence W orked Abroad and Subtype in Returned Laborers in Shandong Province

SU Sheng—li．XING Hui; LIU Chuan—xin; PAN Pin—liang; HUANG Tao FU Ji-hua; ZHAO Shi-li; SHAO Yi—ming

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February 2001

SU Sheng—li．XING Hui, LIU Chuan—xin, PAN Pin—liang, HUANG Tao FU Ji-hua, ZHAO Shi-li, SHAO Yi—ming, Analysis of HIV-1 Sequence W orked Abroad and Subtype in Returned Laborers in Shandong Province[J]. Virologica Sinica, 2001, 16(1): 34-39.

Citation: SU Sheng—li．XING Hui, LIU Chuan—xin, PAN Pin—liang, HUANG Tao FU Ji-hua, ZHAO Shi-li, SHAO Yi—ming, . Analysis of HIV-1 Sequence W orked Abroad and Subtype in Returned Laborers in Shandong Province .VIROLOGICA SINICA, 2001, 16(1) : 34-39.

山东省归国劳务人员中HIV．1感染毒株的序列特征和亚型分析

1.
1山东省卫生防疫站，山东济南250014
2.
生部艾滋病预防与控制中心、北京100050

摘要

为了解自1992年以来，山东省归国劳务人员HIV．1感染毒株的分子漉行病学特征。我们采集了11份确认为HIV-1感染者的垒血分离单核细胞(PBMC)，提取前病毒DNA．用套式PCR扩增HIV一1膜蛋白(删 )基因，井对其 C2-V3区进行序列测定和分析。结果表明：l0名归国劳工人员及1名归国劳工配偶的样本经序列测定和基因分析发觋了HIV-1的4种基因亚型：A、B、C、E亚型。其中C亚型7名，B亚型2名，A和E亚型各1名。由此可见；山东省归国劳务人员LI1 HIV一1毒株的感染情况软复杂．通过计算发现这些毒株间的基因离散率相差较大。提示应加强归国劳务人员的教育、检测和控制防其它国家毒株传人。
- HIV-1
- , 归国劳工
- , 序列测定
- , 亚型
- , 系统树

Analysis of HIV-1 Sequence W orked Abroad and Subtype in Returned Laborers in Shandong Province

1.
Shandong Provincial Health and Anti—epidemic Station，Jinan 250014，China

Abstract

To understand the molecular epidemiologica[characteristic of HIV-1 virus strain in laborers worked abroad in Shandong province since 2992，11 specimens of peripheral blood of identified HIV infected eases were sampled and PBM C was separated by Ficoll density gradient centrifuge． The pre— virus DNA was extracted and the membrane protein gene enzt DNA was multiplied by nested PCR， and then the sequence of env C2一V3 region*as measured and analyzed．The results indicated that 10 labo rers worked abroad and 1 female—spouse of a labo rer worked abroad weAiTe found catching HIV一1 A ， B ，C，E subtype by gene sequencing and analyzing，with subtype C 7，subtype B 2，subtype A and E 1 respectively Thus it can be seen that the infection of HIV virus strain in the 1aborers worked abroad was complex The gene variation rate of these virus strains was greatly different．It is suggest— ed that we strengthen the detection and control of the 1aborers worked abroad to prevent the spread of the virus strains from other countries
- HIV一1
- , Laborers worked abmad
- , DNA sequence measurement
- , Subtype
- , Systematic tree

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Analysis of HIV-1 Sequence W orked Abroad and Subtype in Returned Laborers in Shandong Province

1. Shandong Provincial Health and Anti—epidemic Station，Jinan 250014，China

Abstract: To understand the molecular epidemiologica[characteristic of HIV-1 virus strain in laborers worked abroad in Shandong province since 2992，11 specimens of peripheral blood of identified HIV infected eases were sampled and PBM C was separated by Ficoll density gradient centrifuge． The pre— virus DNA was extracted and the membrane protein gene enzt DNA was multiplied by nested PCR， and then the sequence of env C2一V3 region*as measured and analyzed．The results indicated that 10 labo rers worked abroad and 1 female—spouse of a labo rer worked abroad weAiTe found catching HIV一1 A ， B ，C，E subtype by gene sequencing and analyzing，with subtype C 7，subtype B 2，subtype A and E 1 respectively Thus it can be seen that the infection of HIV virus strain in the 1aborers worked abroad was complex The gene variation rate of these virus strains was greatly different．It is suggest— ed that we strengthen the detection and control of the 1aborers worked abroad to prevent the spread of the virus strains from other countries