Cloning and Sequencing of M ovement Protein Gene of Cymbidium M osaic Virus

LIU Zhi-xin; WU Hao．PAN Jun-song; ZHENG Xue-qin

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February 2001

LIU Zhi-xin, WU Hao．PAN Jun-song, ZHENG Xue-qin and Cloning and Sequencing of M ovement Protein Gene of Cymbidium M osaic Virus[J]. Virologica Sinica, 2001, 16(1): 51-54.

Citation: LIU Zhi-xin, WU Hao．PAN Jun-song, ZHENG Xue-qin, . Cloning and Sequencing of M ovement Protein Gene of Cymbidium M osaic Virus .VIROLOGICA SINICA, 2001, 16(1) : 51-54.

建兰花叶病毒运动蛋白基因克隆及序列分析

1.
中国热带农业科学院热带作物生物技术国家重点实验室．海南海口 57110]

摘要

从建兰花叶病毒(CyMV)石斛兰分离物中提取病毒RNA、用反转录—— 聚合酶链式反应(RT．PCR)方法获得约500 bp的运动蛋白箍因片断，插入pGEM T载体克隆并测序。序列分析表明，该基因片断由474个核苷酸组成，和CyMV美国夏威爽讣离物、新加坡分离物相应基因核甘酸序刊分别具有97 8％同源性{根据核酸序列推导该片断含有3个部分重叠的开放阅读框架(ORF)，分别编码14kD、12 kD和10 kD的多肽。
- 建兰花叶病毒：丝动蛋白
- , 基因克隆
- , 序列分析

Cloning and Sequencing of M ovement Protein Gene of Cymbidium M osaic Virus

1.
NationalKeyBiotechnologyLaboratory r TropicalCrops，Haikou 571101，China

Abstract

The viral RNA was extracted from purified cymbidium mosaic virus(CyMV)isolated from Oendrobium orchid cultivated in Hainan island．The gene of the movement protein(MP)was amplifled by mealls of reverse transcription—polymerase chain reaction(RT．PCR)，and cloned into pGEM T easy vector． Sequence analysis showed that the gene fragment contained 3 open reading frames (ORbs)which may be encoding 14 kD、12 kD and 10 kD peptides The nucleotide sequence of the doned gene~agment shared 97．8％ homology with the MP genes of CyMV isolated from orchids cultivated in Hawaii and Sing apore
- Cymbidium mosaic virus
- , Movement protein
- , Gene cloning

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Cloning and Sequencing of M ovement Protein Gene of Cymbidium M osaic Virus

1. NationalKeyBiotechnologyLaboratory r TropicalCrops，Haikou 571101，China

Abstract: The viral RNA was extracted from purified cymbidium mosaic virus(CyMV)isolated from Oendrobium orchid cultivated in Hainan island．The gene of the movement protein(MP)was amplifled by mealls of reverse transcription—polymerase chain reaction(RT．PCR)，and cloned into pGEM T easy vector． Sequence analysis showed that the gene fragment contained 3 open reading frames (ORbs)which may be encoding 14 kD、12 kD and 10 kD peptides The nucleotide sequence of the doned gene~agment shared 97．8％ homology with the MP genes of CyMV isolated from orchids cultivated in Hawaii and Sing apore