摘要：对重组痘苗病毒和重组杆状病毒表达的狂犬病毒NP及原代地鼠肾细胞培养的狂犬病毒核衣壳蛋白(RNP) 先经Sephar~eCI 4B分f筛柱初步提纯，再以抗狂犬病毒NP McAB 2C12．Sephar~e 4B亲和层析柱纯化分离．经 El [SA．SDS-PAGE电泳和Western—Blot分析证实 获得了高纯度和免疫反应性的NP和RNP 以相同剂量的纯化 蛋白免疫小鼠．RNP和『II『种蓐组NP均可诱生特异的抗NP抗体，三种蛋白问无明显差异；狂犬病毒CVS株攻击保 护实验结果显示，三种 免疫的小鼠存活率约为50％ ；两种重组NP的免疫反应性和免疫原性与天然狂犬病毒 RNP相似。

Abstract：In order tO establish the optimum condition for purification of the nucleopmtein(NP)of rabies virus by immuno0finity chromatography，the efficient and non-danatulative eluants(Mg-d)Ⅵ忸s obtained by using ELISA elution model；furthermore．it didn’t damage the activity of NP．Two kind of NPs,expressed by Te— combimnt vacclnia virus(rVac—N)and~,o3mbinant haculovirus(BRN)，were purified by a Sepharose CL 413 column and a 2C12一Sepham~e 4B oolurrm ByWestern-blot and SDS-PAGE．highpurityandgood antigenie,~ intact NPs were identified． purified ribonucleopmtein(RNP) rabies virus 5aG strain was also 0brained． Aftel"immunized with NP and RNP．mice developed a strong anti-nudeoproteln response and were protected against a lethal chaHenge of rabies virus CVS strain There were not diference been observed araong the mice immunized with different purified protein These data indicate that the NPs are antigenical and immunagenical oomparabletothe authentm rabies RNP aridtherefore present a potantial sour~ ofan dk t ，safeand e∞ nomical subunit vaccine