Expression of P 19 Gene of Avian Leukosis Virus in Escherichia coli

LIU Gong; ZHAO Zhen-fen; LIU Fu-an

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February 2001

LIU Gong, ZHAO Zhen-fen and LIU Fu-an. Expression of P 19 Gene of Avian Leukosis Virus in Escherichia coli[J]. Virologica Sinica, 2001, 16(1): 78-80.

Citation: LIU Gong, ZHAO Zhen-fen, LIU Fu-an. Expression of P 19 Gene of Avian Leukosis Virus in Escherichia coli .VIROLOGICA SINICA, 2001, 16(1) : 78-80.

禽白血病病毒p19基因末端片段在大肠杆菌中的表达

1.
1华南农业大学动物医学系，广东广州510640~ 2．郑州牧业工程高等专科学校．河南郑州450008

摘要

根据禽白血病病毒(A1 v)p19基因末端序列台成一条82 bp的积链DNA片段，将其克隆到表达质粒pGE． MEX一1中．序列分析结果与设计的相符。重组表达质粒转化的大肠杆菌BL21(DE3)经IPTG诱导后产生34kD融台表达产物，与理论值相符；Western．blot分析表明该表达产物能与免抗ALV血清发生反应。
- 禽白血病病毒
- , P19基因
- , 克隆
- , 原核表达

Expression of P 19 Gene of Avian Leukosis Virus in Escherichia coli

1.
1 Department of Veterinary Medicine．South China A Univ Guangzhou 510640，Ch ina 2 Zhengzhou Animal Husbandry College，Zhengzhou 450008，China

Abstract

Abstract：Based on avian 1eukosis virus(ALV)p19 gene termina1 nucleotide sequence，a 82 bp dou— ble．stranded DNA fragment was chemically synthesized and cloned into the expression eect~r pGE— M EX．1 The sequencing result indicated that the cloned fragmentⅥw a correct version of the one de— signed both in nucleotide sequence and in its open reading frame．The recombinant was used to transfo／ Tn E．∞“ BL21(DE3) The cloned fragment was expressed as a fused protein with T7 gene 10 leader peptide and was shown to be 34 kD in size on SDS—PAGE ge1 when induced with l mmol／L IPTG．The expression product was able to bind immunologically to rabbit anti—ALV serum in W est— ern-blot assay and is being tested to differentiate exogenous from endogenous ALV ．
- Avian 1eukosis virus
- , p19 gene
- , C1oning
- , Prokaryotic expression

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Expression of P 19 Gene of Avian Leukosis Virus in Escherichia coli

1. 1 Department of Veterinary Medicine．South China A Univ Guangzhou 510640，Ch ina 2 Zhengzhou Animal Husbandry College，Zhengzhou 450008，China

Abstract: Abstract：Based on avian 1eukosis virus(ALV)p19 gene termina1 nucleotide sequence，a 82 bp dou— ble．stranded DNA fragment was chemically synthesized and cloned into the expression eect~r pGE— M EX．1 The sequencing result indicated that the cloned fragmentⅥw a correct version of the one de— signed both in nucleotide sequence and in its open reading frame．The recombinant was used to transfo／ Tn E．∞“ BL21(DE3) The cloned fragment was expressed as a fused protein with T7 gene 10 leader peptide and was shown to be 34 kD in size on SDS—PAGE ge1 when induced with l mmol／L IPTG．The expression product was able to bind immunologically to rabbit anti—ALV serum in W est— ern-blot assay and is being tested to differentiate exogenous from endogenous ALV ．