Immunoreaetivity Studies of Various HEV Antigen with Anti-HEV IgM of the Sear from Patients Infected with Hepatitis E Virus

RONG Ouang-ya; ZhOU ji—wen; LEI Li; ZHAO Gui—lan; SUN Jie

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April 2001

RONG Ouang-ya, ZhOU ji—wen, LEI Li, ZHAO Gui—lan and SUN Jie. Immunoreaetivity Studies of Various HEV Antigen with Anti-HEV IgM of the Sear from Patients Infected with Hepatitis E Virus[J]. Virologica Sinica, 2001, 16(2): 128-130.

Citation: RONG Ouang-ya, ZhOU ji—wen, LEI Li, ZHAO Gui—lan, SUN Jie. Immunoreaetivity Studies of Various HEV Antigen with Anti-HEV IgM of the Sear from Patients Infected with Hepatitis E Virus .VIROLOGICA SINICA, 2001, 16(2) : 128-130.

不同戊肝抗原检测抗-HEV IgM 反应性研究

1.
北京解放军第302医院临床诊断试剂研究中心，北京1043039

摘要

目的比较不同戊肝抗原检测抗一HEV lgM反应性。方法用HEV E30、E42、E33合成肺和HEV ORF-2重组抗原建立酶免疫试验(EtA)检测肝病患者和健康人群中抗一HEV IgM。结果60份抗一HEV 阳性血清中．用E30、 E42、E33及重组抗原包被检测抗．HEVIgM．阳性率分别为76．6％，26 6％，18 3％．66．7％。用E30抗原进一步检测戊肝急性期及恢复期血清，抗HEV IgM 阳性率为90％及3 3％。结论 HEV E30为抗原的EIA特异性强、灵敏度高，是戊型肝炎早期诊断实用可靠的方法。
- 戊型肝炎病毒
- , 合成肽
- , 重组抗原

Immunoreaetivity Studies of Various HEV Antigen with Anti-HEV IgM of the Sear from Patients Infected with Hepatitis E Virus

1.
Center for DiagnosticReagents，302 Hospital of LA，Belting 100039，China

Abstract

Objective To compare the Iramunoreactivity of various HEV Antigen with An ti—HEV IgM Methods Solid-phase erazyrne immunoassay(EIA )vcas developed for detecting anti HEV IgM by using synthetic peptides E30，E42，E33，and recombinant antigen from HEV ORF一2 Results Of 60 anti-HEV positive sera by using E30，E42，E33 and recombina nt antigen as coating antigen，Anti— HEV IgM positive rates were 76．7％．26，6％．18 3％ and 66 7％ respectively InAcute-phase and convalescence—phase sera of the patients with Hepa titis E．An ti—HEV IgM positive rate was 90％ and 3 3％ respectively．Condusions The HEV E30一based EIA will be very useful in the early diagnosis of Hepatitis E．
- Hepatitis E Virus
- , Synthetic peptides
- , Recombinant antigen

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Immunoreaetivity Studies of Various HEV Antigen with Anti-HEV IgM of the Sear from Patients Infected with Hepatitis E Virus

1. Center for DiagnosticReagents，302 Hospital of LA，Belting 100039，China

Abstract: Objective To compare the Iramunoreactivity of various HEV Antigen with An ti—HEV IgM Methods Solid-phase erazyrne immunoassay(EIA )vcas developed for detecting anti HEV IgM by using synthetic peptides E30，E42，E33，and recombinant antigen from HEV ORF一2 Results Of 60 anti-HEV positive sera by using E30，E42，E33 and recombina nt antigen as coating antigen，Anti— HEV IgM positive rates were 76．7％．26，6％．18 3％ and 66 7％ respectively InAcute-phase and convalescence—phase sera of the patients with Hepa titis E．An ti—HEV IgM positive rate was 90％ and 3 3％ respectively．Condusions The HEV E30一based EIA will be very useful in the early diagnosis of Hepatitis E．