DNA Immunization of Hepatitis E Virus(HEV)

LU ng—mei; CHEN Er-jla; XIE Tian-hor~; ZHUANG Jun—ying．LIU Yong LI Chun—hong; SUN Mao-sheng”; DAI Chang—bai

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April 2001

LU ng—mei, CHEN Er-jla, XIE Tian-hor~, ZHUANG Jun—ying．LIU Yong LI Chun—hong, SUN Mao-sheng”, DAI Chang—bai, DNA Immunization of Hepatitis E Virus(HEV)[J]. Virologica Sinica, 2001, 16(2): 131-134.

Citation: LU ng—mei, CHEN Er-jla, XIE Tian-hor~, ZHUANG Jun—ying．LIU Yong LI Chun—hong, SUN Mao-sheng”, DAI Chang—bai, . DNA Immunization of Hepatitis E Virus(HEV) .VIROLOGICA SINICA, 2001, 16(2) : 131-134.

戊型肝炎病毒DNA免疫的研究

1.
中国医学科学院中国协和医科大学医学生物学研究所，云南昆明 650118

摘要

利用PCR方法获得1163 bp的戊型肝炎(HepatitisEVirus．HEV)开放读码框架(0 nReadingFrame．ORF) ORF2之3’大片段和369bpORF3的完整片段．分别克隆到真棱表达载体pcDNA3中．构建两种含有HEV主要抗原表位的质粒DNA：pcE2和poE3．分别或混合免疫Swiss小鼠三次(0时，第2周．第4局)．观察其在小鼠体内诱发的体液免疫应答。ELISA检测结果表明，pcE2和pcE3在小鼠体内均可诱导出一定水平的HEV IgG抗体．且在第三次免疫接种两周后，100％的小鼠抗体阳转。与两种质粒单独免疫相比两者同时注射的抗体水平较高本研究为HEV DNA疫苗的研究打下一定基础。
- DNA免疫
- , 戊型肝炎病毒
- , 体液免疫

DNA Immunization of Hepatitis E Virus(HEV)

1.
Institute of Medicai Biology，CAMS／PUMC，Kunming 6501 18．China

Abstract

Two plasmid constructs，pcE2 and peE3，containing 3’fragment of open reading frame 2 (ORF2，1163 bp)of hepatitis E virus(HEV)and full—length ORF3(369 bp)．were injected into bi— lateral tibiaLs of Swiss mice respectively．for three times(0，2 and 4 weeks)and observed the HEv IgG by ELIsA HEV IgG was induced after the injection of pcE2 or pcE3 or both，and the percentage of seraconversion was i00％ after two weeks of the third iniection．Compared with injection of either construct，the antibo dy titers were higher in the group with combined injection of two constructs．
- DNA immunization
- , Hepatitis E Virus：Humored response

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

DNA Immunization of Hepatitis E Virus(HEV)

1. Institute of Medicai Biology，CAMS／PUMC，Kunming 6501 18．China

Abstract: Two plasmid constructs，pcE2 and peE3，containing 3’fragment of open reading frame 2 (ORF2，1163 bp)of hepatitis E virus(HEV)and full—length ORF3(369 bp)．were injected into bi— lateral tibiaLs of Swiss mice respectively．for three times(0，2 and 4 weeks)and observed the HEv IgG by ELIsA HEV IgG was induced after the injection of pcE2 or pcE3 or both，and the percentage of seraconversion was i00％ after two weeks of the third iniection．Compared with injection of either construct，the antibo dy titers were higher in the group with combined injection of two constructs．