Comparison of Producing Dendrolimus punctatus Cytoplasm ic Polyhedrosis Virus in Substitutive Host Insect

MA Yong-ping; MENG Xiao—lin ．HU Rong; XU Jing—ping

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April 2001

MA Yong-ping, MENG Xiao—lin ．HU Rong, XU Jing—ping and Comparison of Producing Dendrolimus punctatus Cytoplasm ic Polyhedrosis Virus in Substitutive Host Insect[J]. Virologica Sinica, 2001, 16(2): 155-160.

Citation: MA Yong-ping, MENG Xiao—lin ．HU Rong, XU Jing—ping, . Comparison of Producing Dendrolimus punctatus Cytoplasm ic Polyhedrosis Virus in Substitutive Host Insect .VIROLOGICA SINICA, 2001, 16(2) : 155-160.

替代宿主增殖松毛虫质型多角体病毒的比较研究

1.
武汉大学病毒研究所．湖北武汉430072

摘要

银纹夜蛾幼虫(Argyrogramma agnata)对松毛虫CPV(DpCPV)十分敏感，本文对两种不同的宿主增殖松毛虫CPV作了比较．屯镜证实用替代宿主增殖的DpCPV与原毒株多角体(CPB)和病毒粒子的形态完全一致。3％ PAGE分析二者的RNA图谱基本一致．有大小相同的2 98×10‘一0．66 X 10 道尔顿的10条带．用它增殖的 DpCPV(Aa-DpCPV)对松毛虫有相当的毒力．每头3龄幼虫病毒产量平均为2 5 X10 CPB。
- 松毛虫
- , 质型多角体病毒
- , 替代宿主
- , 银纹夜蛾
- , 基因组

Comparison of Producing Dendrolimus punctatus Cytoplasm ic Polyhedrosis Virus in Substitutive Host Insect

1.
Institute of Virology，Wuhan University．Wuhan 430072．China

Abstract

Argyrogramma agnata has been selected as a substitutive host insect for producing Den drolimus punctatus Cytoplasmic Polyhedmsis Virus(DpCPV)，In our experiment．it is very suscepti— ble to DpCPV．The DpCPV produced in A．agnata is designated Aa-DpCPV The cytoplasmic poly— hedra body(CPB)．the virion size and the shape of Aa—DpCPV are same as that of its original DpCPV (DpCPV-W 1984) The RNA bands of Aa-DpCPV and DpCPV—W 1984 al1 have 10 RNA segments re— spectively in 3％ PAGE．which molecular weights ranged in size from 2 98×10。to 0 66× 10。Dal— ton．Aa—DpCPV has the saro．e strong toxicity as that of DpCPV-W 1984(from D punctatus)tO D． punctatus(Walker)larva So it cai2 he applied tO the pine caterpillar contro1．The DpCPV yield in A．agnata iS 2．5 x 10 CPB／larva
- Dendrolimus punctatus
- , Cytoplasmic Polyhedrosis Virus
- , Substitutive host insect
- , Argy— rogramma agnata
- , Genome

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1.
沈阳化工大学材料科学与工程学院沈阳 110142

Comparison of Producing Dendrolimus punctatus Cytoplasm ic Polyhedrosis Virus in Substitutive Host Insect

1. Institute of Virology，Wuhan University．Wuhan 430072．China

Abstract: Argyrogramma agnata has been selected as a substitutive host insect for producing Den drolimus punctatus Cytoplasmic Polyhedmsis Virus(DpCPV)，In our experiment．it is very suscepti— ble to DpCPV．The DpCPV produced in A．agnata is designated Aa-DpCPV The cytoplasmic poly— hedra body(CPB)．the virion size and the shape of Aa—DpCPV are same as that of its original DpCPV (DpCPV-W 1984) The RNA bands of Aa-DpCPV and DpCPV—W 1984 al1 have 10 RNA segments re— spectively in 3％ PAGE．which molecular weights ranged in size from 2 98×10。to 0 66× 10。Dal— ton．Aa—DpCPV has the saro．e strong toxicity as that of DpCPV-W 1984(from D punctatus)tO D． punctatus(Walker)larva So it cai2 he applied tO the pine caterpillar contro1．The DpCPV yield in A．agnata iS 2．5 x 10 CPB／larva