CHENG Ye, CHEN Jiong‘一, ZHENG Tao .YANG ]Jan—ping, CHEN ]ianpins and Maize Dwarf Mosaic Disease Occurred in Hangzhou Isolate Caused by Sugarcane M osaic Virus[J]. Virologica Sinica, 2001, 16(2): 170-174.
Citation: CHENG Ye, CHEN Jiong‘一, ZHENG Tao .YANG ]Jan—ping, CHEN ]ianpins, . Maize Dwarf Mosaic Disease Occurred in Hangzhou Isolate Caused by Sugarcane M osaic Virus .VIROLOGICA SINICA, 2001, 16(2) : 170-174.

杭州地区发生的玉米花叶病由甘蔗花叶病毒引起

  • 从杭州地区呈现玉米矮花叶典型症状的玉米病组织中提纯得到大量线状病毒粒子 大多数长度为750 nm 病组织中含有大量风轮状内古体和板状集结体。病毒外壳蛋白为33 6 kD。病毒RNA1 3’端序列(1 8 kb)与甘蔗 花叶病毒(SCMV)同源性最高.达71 5%~99 1%,与高粱花叶病毒(srMv)同源性砍之,为67.8%~68 5%,与玉 米矮花叶病毒(MDMV)同源性最低.仅为38 4% ~48 4%.从而初步认为此病害由SCMV引起。根据已发表的 SCMV外壳蛋白氯基酸序列作亲缘性分析,表明SCMV可分为美国、南非、澳大利亚;德国和中国三大类。

Maize Dwarf Mosaic Disease Occurred in Hangzhou Isolate Caused by Sugarcane M osaic Virus

  • Recently maize dwarf mosaic disease WaS occurred on maize crop seriously in large scale in Hangzhou district.Purified preparations from the infected maize leaves~ontained numerous filamen— t0us virus particles of e 750 nm in leng th.Cells of infected plants contained typical pinwheels and lam— inated aggregates.The coat protein of the virus was 33.6 kD A 1.8 kb fragment of 3’一terminus of the viral RNA was amplified by RT—PCR,cloned and its sequence was determined。Sequence compar— isons showed that it shared 71 5% -99 1% homology with isolates of sugarcane mosaic virus.67。8% ~ 68.5% with sorghum mosaic virus and 38。4% ~48.4% with ma ize dwarf mosaic virus,indicating that the pathogen of this disease on maize in Hangzhou was sugarcane mosaic virus.In addition.the relationships of sugarcane mosaic virus isolates from different origins all OVer world were discussed based 0n coat protein sequences

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    Maize Dwarf Mosaic Disease Occurred in Hangzhou Isolate Caused by Sugarcane M osaic Virus

    • 1. 1.VirologyLaboratory,ZhejiangAcademy ofAgricultural Sciowes,Hangzhou 310021。China
    • 2. Department of Biological Science and Biotechnology,Zhejiang University。Hangzhou 310027。China

    Abstract: Recently maize dwarf mosaic disease WaS occurred on maize crop seriously in large scale in Hangzhou district.Purified preparations from the infected maize leaves~ontained numerous filamen— t0us virus particles of e 750 nm in leng th.Cells of infected plants contained typical pinwheels and lam— inated aggregates.The coat protein of the virus was 33.6 kD A 1.8 kb fragment of 3’一terminus of the viral RNA was amplified by RT—PCR,cloned and its sequence was determined。Sequence compar— isons showed that it shared 71 5% -99 1% homology with isolates of sugarcane mosaic virus.67。8% ~ 68.5% with sorghum mosaic virus and 38。4% ~48.4% with ma ize dwarf mosaic virus,indicating that the pathogen of this disease on maize in Hangzhou was sugarcane mosaic virus.In addition.the relationships of sugarcane mosaic virus isolates from different origins all OVer world were discussed based 0n coat protein sequences

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