Detection of Trv DNA in Various Populations by Polymerase Chain Reaction-microplate Hybridization

GUO Nai—zhou．MA Da; WANG Wan-xlang

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June 2001

GUO Nai—zhou．MA Da and WANG Wan-xlang. Detection of Trv DNA in Various Populations by Polymerase Chain Reaction-microplate Hybridization[J]. Virologica Sinica, 2001, 16(3): 208-211.

Citation: GUO Nai—zhou．MA Da, WANG Wan-xlang. Detection of Trv DNA in Various Populations by Polymerase Chain Reaction-microplate Hybridization .VIROLOGICA SINICA, 2001, 16(3) : 208-211.

PCR微孔板杂交法检测不同人群TTV DNA

郭乃洲 ,
王万相

1.
江苏省盐城市第一人民医院检验科．江苏盐城224001

摘要

根据报道的Trv全序列设计引物和探针，建立眦’微孔板杂交法，检测81例正常人群、92例职业献血员123 例甲一庚型肝炎、32例非甲一庚型肝炎、48例原发性肝癌患者的TⅣ DNA。结果表明Trv在以上五种人群中的阳性卓分别为3 7％、4．3％、21．1％、28．1％、52．O％，前者与后三者比较有显著性差异(P0．O5)，Trv台并HBV二重感染占重叠感染的54．0％。这揭示不同人群均存在Trv感染．正常人群和职业献血员存在健康携带状态，甲一庚型肝炎和非甲一庚肝炎病人为高危人群，Trv可与各型肝炎存在重叠感染，Trv除经血传播外．存在其它传播途径． Tnt感染与ALT及TBⅢ 的升高密切相关。
- TⅣ
- , PCR_微孔板杂交
- , TⅣ DNA
- , 肝炎

Detection of Trv DNA in Various Populations by Polymerase Chain Reaction-microplate Hybridization

1.
The first people's hospital of Yancheng,Jiangsu 224001,China

Abstract

TTV DNA were detected by polymerase chain reaction-microplate bybridization.The results showed that the positive rate of TTV DNA in 81 normal samples,92 blood donation samples,123 A to G hepatitis,32non-A to G hepatitis samples and 48 proimitive hepatic-carcinoma samples was 3.7%?4.3%?21.1%? 28.1% ?52.0%,respectively.There were significant difference between the normal?the hepatitis group and the primitive hepatic-carcinoma group.Among multiinfection,the infection rate of TTV with HBV was as high as 54.0%.The data showed that TTV infection was very popular in different groups.Some of the normal and blood donation group infected with TTV could become virus carrier.And the hepatits patients were high risk group.In addition,TTV may be transmitted by other means besides blood.TTV infection has close relationship with the increase of ALT and TBIL.
- TTV
- , Polymerase chain reaction-Microplate hybridizaficn
- , TTV DNA
- , Hepatitis．

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Detection of Trv DNA in Various Populations by Polymerase Chain Reaction-microplate Hybridization

1. The first people's hospital of Yancheng,Jiangsu 224001,China

Abstract: TTV DNA were detected by polymerase chain reaction-microplate bybridization.The results showed that the positive rate of TTV DNA in 81 normal samples,92 blood donation samples,123 A to G hepatitis,32non-A to G hepatitis samples and 48 proimitive hepatic-carcinoma samples was 3.7%?4.3%?21.1%? 28.1% ?52.0%,respectively.There were significant difference between the normal?the hepatitis group and the primitive hepatic-carcinoma group.Among multiinfection,the infection rate of TTV with HBV was as high as 54.0%.The data showed that TTV infection was very popular in different groups.Some of the normal and blood donation group infected with TTV could become virus carrier.And the hepatits patients were high risk group.In addition,TTV may be transmitted by other means besides blood.TTV infection has close relationship with the increase of ALT and TBIL.