Isolation and Adaptation of HAV on M EK Cells and Vero Cells

JING Shen—rong; JianG Shu—de; YU feng; WANG Yan

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June 2001

JING Shen—rong, JianG Shu—de, YU feng and WANG Yan. Isolation and Adaptation of HAV on M EK Cells and Vero Cells[J]. Virologica Sinica, 2001, 16(3): 212-214.

Citation: JING Shen—rong, JianG Shu—de, YU feng, WANG Yan. Isolation and Adaptation of HAV on M EK Cells and Vero Cells .VIROLOGICA SINICA, 2001, 16(3) : 212-214.

甲肝病毒在猴胚肾细胞和Vero细胞上的分离与适应

1.
中国医学科学院中国协和医科大学医学生物学研究所，云南昆明650118

摘要

使用恒河猴胚肾(加强)细胞从临床标本中分离和适应了甲肝病毒w和X，通过阻断实验、中和实验、免疫荧光和免疫电镜实验、RT-PCR对其进行特异性鉴定，证明是甲肝病毒。w株和x株第7代在MEK细胞上的抗原滴度分别为1：512、1：1024，感染滴度(1ogTCIDxJmL)分别为8．17、8．50。只有分离株w可以适应于细胞，第6代21d 抗原滴度为1：256，感染滴度( I蜘／rnL)为8．o0。
- 甲肝病毒
- , MEK细胞
- , Vem细胞
- , 分离
- , 适应

Isolation and Adaptation of HAV on M EK Cells and Vero Cells

1.
中国医学科学院中国协和医科大学医学生物学研究所，云南昆明650118

Abstract

HAV strains W and X were isolated and adapted to Rhesus monkey embryo kidney(MEK) cells from feces of hepatitis A patients,and identified to be HAV with blocking experiment,neutralizing experiment,indirect immunofluorescence (IF),immune electron microscopy(IEM) and RT-PCR.Antigenic titers of strains W and X P7 on MEK cells were 1∶512,1∶1024,respectively and infective titers(logTCID 50 /mL)of that were 8.17 ,8.50 respectively.Only the isolate W was adapted to Vero cells.Antigenic titer of adapted strain W P6 on Vero cells on 21th day was 1∶256 and infective titer(logTCID 50 /mL)was 8.00.
- HAV
- , MEK cell
- , Veto cell
- , Isolation
- , Adaptation

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Isolation and Adaptation of HAV on M EK Cells and Vero Cells

1. 中国医学科学院中国协和医科大学医学生物学研究所，云南昆明650118

Abstract: HAV strains W and X were isolated and adapted to Rhesus monkey embryo kidney(MEK) cells from feces of hepatitis A patients,and identified to be HAV with blocking experiment,neutralizing experiment,indirect immunofluorescence (IF),immune electron microscopy(IEM) and RT-PCR.Antigenic titers of strains W and X P7 on MEK cells were 1∶512,1∶1024,respectively and infective titers(logTCID 50 /mL)of that were 8.17 ,8.50 respectively.Only the isolate W was adapted to Vero cells.Antigenic titer of adapted strain W P6 on Vero cells on 21th day was 1∶256 and infective titer(logTCID 50 /mL)was 8.00.