Construction of cDNA Clone and Nucleotde Sequence of RNA3 of Cucumber Mosaic Virus Isolate Xb from Banana

WANG Hai-he．XIE Iian—hui ．LIN Qi-ying

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June 2001

WANG Hai-he．XIE Iian—hui ．LIN Qi-ying, Construction of cDNA Clone and Nucleotde Sequence of RNA3 of Cucumber Mosaic Virus Isolate Xb from Banana[J]. Virologica Sinica, 2001, 16(3): 252-256.

Citation: WANG Hai-he．XIE Iian—hui ．LIN Qi-ying, . Construction of cDNA Clone and Nucleotde Sequence of RNA3 of Cucumber Mosaic Virus Isolate Xb from Banana .VIROLOGICA SINICA, 2001, 16(3) : 252-256.

黄瓜花叶病毒香蕉株系(CMV—Xb)RNA3 eDNA的克隆和序列分析

1.
福建农业大学植物病毒研究所，福建省植物病毒学重点实验室，福建福州350002

摘要

通过lIT-PeR方法，设计两对引物，克隆了黄瓜花叶病毒香蕉株系(C~,f~-Xb)ITN_43，并进行了棱苷酸和蛋白质水平上的分析。结果表明xb株系I1NA3全长2205nt，具有两个蛋白编码阅读框架(O~dr)．其中5’端(97—936nt)编码一个279粕的3a蛋白；3’端(1225—1871nt)编码一个218aa的CP蛋白。5’非编码区域为96Il【；基因间隔区(IR)长 288nt；3’NR含有324nt。通过与亚组Ⅱ其它株系RNA3棱苷酸和所编码产物推导的氨基酸序列分析发现，亚组Ⅱ株系无论在编码区还是非编码区的核苷酸同源性都相对较高；亚组Ⅱ株系在进化过程中具有连续性。
- 黄瓜花叶病毒香蕉株系
- , IINA3
- , 全长cDNA克隆
- , 序列分析

Construction of cDNA Clone and Nucleotde Sequence of RNA3 of Cucumber Mosaic Virus Isolate Xb from Banana

1.
Institute of plant virology,Fujian Agricultural University,Key Lab of Plant Virology of Fujian Province,Fuzhou 350002.China

Abstract

The nucleotide sequence of RNA3 of a cucumber mosaic virus strain from banana of subgroup Ⅱ(CMV-Xb) was cloned and analyzed at nucleotide and protein level after RT-PCR amplificaion with two pairs of primers.The results showed that CMV-Xb is composed of 2205 nucleotides(nt) with two open reading frames.The one near 5'termini(97～936nt) encodes a 3a protein with 279 amino acids(aa),the other near 3'termini (1225～1871nt)codes for coat protein with 218aa,The 5'non-coding region(NR),intercistron region(IR) and 3'NR are 96nt,288nt and 324nt, respectively.Comparison at the nucleotide and putative amino acid level with other strains in subgroup Ⅱ showed that the nucleotide sequence of Xb is very conservative in the encoding regions as well as non-coding regions.The evolution of RNA3s of subgroup Ⅱ members of CMV is consecutive.
- Cucumber mosaic virus swain from bamna
- , RNA3
- , Full—length eDNA
- , Nucleotide sequence

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Construction of cDNA Clone and Nucleotde Sequence of RNA3 of Cucumber Mosaic Virus Isolate Xb from Banana

1. Institute of plant virology,Fujian Agricultural University,Key Lab of Plant Virology of Fujian Province,Fuzhou 350002.China

Abstract: The nucleotide sequence of RNA3 of a cucumber mosaic virus strain from banana of subgroup Ⅱ(CMV-Xb) was cloned and analyzed at nucleotide and protein level after RT-PCR amplificaion with two pairs of primers.The results showed that CMV-Xb is composed of 2205 nucleotides(nt) with two open reading frames.The one near 5'termini(97～936nt) encodes a 3a protein with 279 amino acids(aa),the other near 3'termini (1225～1871nt)codes for coat protein with 218aa,The 5'non-coding region(NR),intercistron region(IR) and 3'NR are 96nt,288nt and 324nt, respectively.Comparison at the nucleotide and putative amino acid level with other strains in subgroup Ⅱ showed that the nucleotide sequence of Xb is very conservative in the encoding regions as well as non-coding regions.The evolution of RNA3s of subgroup Ⅱ members of CMV is consecutive.