Construction of Gene Library and Localization of Chitinase Gene of Parocneria orienta Nuclear polyhedrovirus

ZHAO Xiao-dong; ZHOU Jian-hua; DING Qing-quan

PDF
Cite
Share

facebook

twitter

google

linkedin

June 2001

ZHAO Xiao-dong, ZHOU Jian-hua and DING Qing-quan. Construction of Gene Library and Localization of Chitinase Gene of Parocneria orienta Nuclear polyhedrovirus[J]. Virologica Sinica, 2001, 16(3): 286-288.

Citation: ZHAO Xiao-dong, ZHOU Jian-hua, DING Qing-quan. Construction of Gene Library and Localization of Chitinase Gene of Parocneria orienta Nuclear polyhedrovirus .VIROLOGICA SINICA, 2001, 16(3) : 286-288.

蜀柏毒蛾核型多角体病毒基因文库的构建及几丁质酶基因的定位

1.
1中国科学武汉病毒研究所．湖北武汉430071
2.
四川省林业科学院．四川成都 610081

摘要

蜀柏毒蛾(Parocner／a。咖Chao)是柏木、桧柏、干头柏等柏科树种的重要食叶害虫¨l J，到目前为止仅存在于我国。蜀柏毒蛾核型多角体病毒 (Parocner／a orienta nuclear polyhedrovims，PaorNPV)[ ] 于1991年被分离，该病毒对蜀柏毒蛾幼虫具有较强毒力，已作为一种新型的生物农药初步应用于柏木林区害虫的防治上¨3 J。对这种病毒已进行了一些研究，包括生物活性测定，形态结构，理化特性，限制性内切酶分析_4J一。为了进一步研究其分子生物学特性，开发我国这种特有的病毒资源，我们构建了部分 Pao’rNPV基因组DNA片段的基因文库，同时以中国棉铃虫单粒包埋核型多角体病毒(HearSN?V)几丁质酶基因作探针，对PaorNPV几丁质酶基因进行了定位，结果报道如下。
- 蜀柏毒蛾核型多角体病毒
- , 基因文库
- , 几丁质酶基因
- , 基因定位

Construction of Gene Library and Localization of Chitinase Gene of Parocneria orienta Nuclear polyhedrovirus

1.
1．Institute of virology，Chinese Academy of Sciences，Wuhan 430071，China 2．Sichuan Academy of sciences，Chendu 610081，China

Abstract

The gene library of Parocneria oriema nuclear polyhedlovims(PaorNPV)was panly constructed by doing$oiriefragnen~ ofBamH I and EcoR I ofP~rNPV genomic DNA into pGEM3Z(f)as a vector．Using the chifinase gene of Hear SNPV probed wlth[a-32p]dCTP，the chifinase gene ofPaorNPV localized OI3_the fragraents of BamH I-D，EcoR[-A，‰ dⅢ一A，Pst LD and Xho tG／H respectively．Atthe time，the fragment ofBamH I-D eontniningthe chiA has been cloned．
- words：Parocneria 0rienta nuclear polyhedrovirus
- , ChitiAIe library
- , Chitinase gene
- , Cve21e loealizafion

References
Proportional views

PDF

Article Metrics

Article views(3980) PDF downloads(861) Cited by(0)

Proportional views

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Construction of Gene Library and Localization of Chitinase Gene of Parocneria orienta Nuclear polyhedrovirus

1. 1．Institute of virology，Chinese Academy of Sciences，Wuhan 430071，China 2．Sichuan Academy of sciences，Chendu 610081，China

Abstract: The gene library of Parocneria oriema nuclear polyhedlovims(PaorNPV)was panly constructed by doing$oiriefragnen~ ofBamH I and EcoR I ofP~rNPV genomic DNA into pGEM3Z(f)as a vector．Using the chifinase gene of Hear SNPV probed wlth[a-32p]dCTP，the chifinase gene ofPaorNPV localized OI3_the fragraents of BamH I-D，EcoR[-A，‰ dⅢ一A，Pst LD and Xho tG／H respectively．Atthe time，the fragment ofBamH I-D eontniningthe chiA has been cloned．