Development of RT-PCR Assay for the De tection of Bluetongue Virus

ZHANG Peng-wei; DONG Charlg-yuan“; TU Pan; GUO Shu-fang; CHENG Xian; YAN Yin-fang

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August 2001

ZHANG Peng-wei, DONG Charlg-yuan“, TU Pan, GUO Shu-fang, CHENG Xian and YAN Yin-fang. Development of RT-PCR Assay for the De tection of Bluetongue Virus[J]. Virologica Sinica, 2001, 16(4): 393-396.

Citation: ZHANG Peng-wei, DONG Charlg-yuan“, TU Pan, GUO Shu-fang, CHENG Xian, YAN Yin-fang. Development of RT-PCR Assay for the De tection of Bluetongue Virus .VIROLOGICA SINICA, 2001, 16(4) : 393-396.

RT-PCR检测蓝舌病毒技术的建立

1.
武汉大学医学院医学病毒研究所．湖北武汉430071

摘要

Using primers complementary to the conserved sequence previously published of BTV genomic dsRNA segment 7,a part of the 5′end of segment 7 was synthesized and amplified by RT PCR method.We adapted this method to test on 12 blood samples suspected to be with BTV and the blood sample of a health sheep as a negative control.The results suggested that eleven of the twelve samples were positive,one was negative.In order to compare the sensitivity and reliability of RT PCR technique,Vero cells were used to isolate BTV from these suspected samples and the results will be a control.The study proved that RT PCR is a rapid,sensitive and precise method for detection and identification of bluetongue virus in clinical samples,animal quarantine and scientific research.
- Bluetongue
- , Virus
- , RT
- , PCR
- , of
- , DsRNA
- , Detection
- , of
- , virus
- , nucleic
- , acid

Development of RT-PCR Assay for the De tection of Bluetongue Virus

1.
武汉大学医学院医学病毒研究所湖北武汉湖北武汉43007

Abstract

Using primers complementary to the conserved sequence previously published of BTV genomic dsRNA segment 7,a part of the 5′end of segment 7 was synthesized and amplified by RT PCR method.We adapted this method to test on 12 blood samples suspected to be with BTV and the blood sample of a health sheep as a negative control.The results suggested that eleven of the twelve samples were positive,one was negative.In order to compare the sensitivity and reliability of RT PCR technique,Vero cells were used to isolate BTV from these suspected samples and the results will be a control.The study proved that RT PCR is a rapid,sensitive and precise method for detection and identification of bluetongue virus in clinical samples,animal quarantine and scientific research.
- Bluetongue Virus RT PCR of DsRNA Detection of virus nucleic acid

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1.
沈阳化工大学材料科学与工程学院沈阳 110142

Development of RT-PCR Assay for the De tection of Bluetongue Virus

1. 武汉大学医学院医学病毒研究所湖北武汉湖北武汉43007

Abstract: Using primers complementary to the conserved sequence previously published of BTV genomic dsRNA segment 7,a part of the 5′end of segment 7 was synthesized and amplified by RT PCR method.We adapted this method to test on 12 blood samples suspected to be with BTV and the blood sample of a health sheep as a negative control.The results suggested that eleven of the twelve samples were positive,one was negative.In order to compare the sensitivity and reliability of RT PCR technique,Vero cells were used to isolate BTV from these suspected samples and the results will be a control.The study proved that RT PCR is a rapid,sensitive and precise method for detection and identification of bluetongue virus in clinical samples,animal quarantine and scientific research.