抽要：最近的报道指出，某些病毒有诱导体外细胞 亡的作用，借以限制病毒的扩散。为探讨HSV．2在体内诱导细胞谰亡的救果及其形态学特点，用HSV-2 333株感染小鼠明道，于感染后不同天数处死动物．取其阴道，固定于10％中性福尔马林，TUNEL末端标记染色显示 亡的细胞，光镜下进行原位观察。结果显示：感染后的第l天粘膜上皮内即出现大量的凋亡细胞，第2天至ll天j弼亡细胞的数量及在上皮内的分布范围达最高水平。早期的拥亡细胞见于感染后所有标本，其核染色质形态及分布似正常细胞 但它被TUNEL标记染成橡黄色：晚期的谰亡细胞亦见于所有标车 其胞棱缩小，染色质浓缩并在核周边集聚，核中心空化。载有拥亡细胞的上皮在阴道牯膜上分布很广，最广的可占全明道上皮的2／3。同时可见HSV一2引起的上皮细胞坏死及疱疹形成，二者均由凋亡细胞包围。 亡细咆不断地由上皮表面脱落至酮遭．未见凋亡小体及眷噬现象。结果提示，HSV 2 333幛阴遭感染可同时诱导细胞坏死及谰亡，细胞捌亡可能在限制病毒产生子代及限{l；I感染区域扩展起重要作用。 Abstract：Recently 8ome reports have shown that some viruses could induce apoptosis of infected cells in uitro to limit their spread．To clarify the effect of HSV-2 strain 333 on induction of apoptosis and its morphological characters in vaginal epithelia the mice were infected with the virus intravagina lly，an d killed at the intervals between day 1-11 post infection(P．I．)．The vaginas weTe taken and fixed in 10％ neutral formalin for paraffinembeding section and TUNEL staining to demonstrate apoptosis． The results were as follows．Numerous apoptotic cells appeared in the epithelia on the first day of in— fection ．the number of the apoptotic cells reached to a high level on day 2—11 P．I．．At early stage of apoptosis，the cell nuclei displayed norma1．but their chrornatin were stained by TUNEL staining． W hen the chromatin condensed and migrated to the periphery．then left a blank space in the center of the nuc ．The apoptotic area spread widely．occupying 1／4·2／4 of the vaginal epithelia At the S；~L,’TLe time，the necrotic areas and herpetic vesicles in the epithelia could be found to restrict only in Solve areas not as widely as the apoptotic areas and were surrounded by the apoptotic areas The apoptotic cells fell off into the vaginal hanen without form ing apoptotic bodies an d phagocyosis．The results showed that the HSV一2 strain 333 could induce apoptosis and necrosis of vaginal mucosal epithelia at the 8an~e time in mice．The apoptos~ might play an impomant role in limiting both the range of viral spreading an dthe production of progenyvirus

Abstract：Recently 8ome reports have shown that some viruses could induce apoptosis of infected cells in uitro to limit their spread．To clarify the effect of HSV-2 strain 333 on induction of apoptosis and its morphological characters in vaginal epithelia the mice were infected with the virus intravagina lly，an d killed at the intervals between day 1-11 post infection(P．I．)．The vaginas weTe taken and fixed in 10％ neutral formalin for paraffinembeding section and TUNEL staining to demonstrate apoptosis． The results were as follows．Numerous apoptotic cells appeared in the epithelia on the first day of in— fection ．the number of the apoptotic cells reached to a high level on day 2—11 P．I．．At early stage of apoptosis，the cell nuclei displayed norma1．but their chrornatin were stained by TUNEL staining． W hen the chromatin condensed and migrated to the periphery．then left a blank space in the center of the nuc ．The apoptotic area spread widely．occupying 1／4·2／4 of the vaginal epithelia At the S；~L,’TLe time，the necrotic areas and herpetic vesicles in the epithelia could be found to restrict only in Solve areas not as widely as the apoptotic areas and were surrounded by the apoptotic areas The apoptotic cells fell off into the vaginal hanen without form ing apoptotic bodies an d phagocyosis．The results showed that the HSV一2 strain 333 could induce apoptosis and necrosis of vaginal mucosal epithelia at the 8an~e time in mice．The apoptos~ might play an impomant role in limiting both the range of viral spreading an dthe production of progenyvirus