Amplification of Dendrolimus puctatus whenshanesis Cytovirus (DpCPV-W)in vitro in Sf21 Cell

MA Yong ping; MENG Xiao—lin; XU Jin—ping

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April 2002

MA Yong ping, MENG Xiao—lin and XU Jin—ping. Amplification of Dendrolimus puctatus whenshanesis Cytovirus (DpCPV-W)in vitro in Sf21 Cell[J]. Virologica Sinica, 2002, 17(2): 137-141.

Citation: MA Yong ping, MENG Xiao—lin, XU Jin—ping. Amplification of Dendrolimus puctatus whenshanesis Cytovirus (DpCPV-W)in vitro in Sf21 Cell .VIROLOGICA SINICA, 2002, 17(2) : 137-141.

文山松毛虫质型多角体病毒在Sf21细胞中离体增殖试验

1.
武汉大学病毒研究所,湖北武汉430072

摘要

摘要：研究了文山松毛虫质型多角体病毒(DpCPV．W)在St21细胞中的离体增殖行为．并进行丁空斑试验．结果显示DpCPV．W 毒株能够在Sf2i细胞中增殖．也能在Sf2i细胞上形成空斑．井能产生形态正常的病毒多角体。在 DpCPV W 中加入基因工程增效蛋白．能显著增加病毒粒子对离I奉细胞的感染率．增幅达145％生物渤l定表明．离体增殖的病毒多角体与虫体增殖的多角体的毒力相当。因此．Sf2i细胞可作为文山松毛虫CPV增殖行为研究的离体细胞系统．也可通过空斑纯化技术对文山松毛虫CPV生产防治的病毒种进行单个病毒粒子的分离纯化。
- 文山松毛虫CpV(DpCPVW)
- , Sf2i细胞
- , 离体
- , 空斑试验
- , 增效蛋白

Amplification of Dendrolimus puctatus whenshanesis Cytovirus (DpCPV-W)in vitro in Sf21 Cell

1.
Institute of virology，Wuhan University．Wuhan 430072．China

Abstract

We have studied amphificatlon of DpCPV W in vitro in Sf21 cell ln this paper．The results showed that DpCPV W could amplify is vitro in Sf2I ce t[and form normal potyhedra Acce~ional TeV eombinant enhancln in medium coutd synergist the infective rate of DpCPV—W in Sf21 cel1 in vitro． Bioassay indicated that the toxici’ty of DpCPV—W polyhedra amplified in Sf2 I cell was as violence a5 the polyhedra amplified in pine caterpillar By using the plaque purfieation technique in Sf21 cell，DpCPV— W could be purified n a single virion clone Ievd
- Dendrolimus punctatus whenshanesis Cytoplasmic Polyhedrosis Virus(DpCPV—W)
- , Sf21 cell
- , in vitro
- , Plaque test
- , Enhancin

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Amplification of Dendrolimus puctatus whenshanesis Cytovirus (DpCPV-W)in vitro in Sf21 Cell

1. Institute of virology，Wuhan University．Wuhan 430072．China

Abstract: We have studied amphificatlon of DpCPV W in vitro in Sf21 cell ln this paper．The results showed that DpCPV W could amplify is vitro in Sf2I ce t[and form normal potyhedra Acce~ional TeV eombinant enhancln in medium coutd synergist the infective rate of DpCPV—W in Sf21 cel1 in vitro． Bioassay indicated that the toxici’ty of DpCPV—W polyhedra amplified in Sf2 I cell was as violence a5 the polyhedra amplified in pine caterpillar By using the plaque purfieation technique in Sf21 cell，DpCPV— W could be purified n a single virion clone Ievd