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Volume 17 Issue 2
April 2002

    DAI Ya, CHEN De, PAN Jie, JIANG Yan, LIU Xing, LU Yin, LIU Mei, DING Chan, CHEN Pu and CAI Bao-xiang. Am plification，Cloning and Sequence Analysis of S1 Genes from Infectious Bronchitis Virus Isolates JS／95／03 and SD／97／01[J]. Virologica Sinica, 2002, 17(2): 172-177.
    Citation: DAI Ya, CHEN De, PAN Jie, JIANG Yan, LIU Xing, LU Yin, LIU Mei, DING Chan, CHEN Pu, CAI Bao-xiang. Am plification，Cloning and Sequence Analysis of S1 Genes from Infectious Bronchitis Virus Isolates JS／95／03 and SD／97／01 .VIROLOGICA SINICA, 2002, 17(2) : 172-177.
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    鸡传染性支气管炎病毒两个地方分离株s1基因的扩增 克隆与序列分析

    • 1.

      1．南京农业大学动物医学院，江苏南京21~095

    • 2.

      江苏册海地区农业科学研究所．江苏盐城2241M32 3 江苏省家畜科学研究所．江苏扬州225003

    • 对IBV肾型毒株JS／95／03和呼吸型毒株SD／97／01的s1全基困进行了扩增、克隆和序列测定．将两毒株的 sl基因序列与l0个参考毒株进行了比较。结果表明，1s／95／03和SD／97／01分剐与M41和H120株亲缘美幕最 近，它们可能是疫苗毒株的变异株。JS／95／03和SD／97／01问的亲缘关系也较近，两者s1蛋白中只有24个氨基酸 的差异，其中有15个位于前130个氯基酸中。第116位氪基酸可能与毒株的致病性有关 对两毒株s1蛋白的二级 结构进行了预测和比较，结果发现，一个或极少数氨基酸的差异即可导致s1蛋自二级结构和抗原性的改变。

    Am plification，Cloning and Sequence Analysis of S1 Genes from Infectious Bronchitis Virus Isolates JS／95／03 and SD／97／01

    • 1.

      College of Veterinary Medicine．Nanjing agriculture University，Nanjing 210095-China

    • 2.

      Institute of Agrlculturecie~i~es in Jiangsu Coastal Area，Yancheng 224002．China

    • Abstract：The entire S1 genes from nephropathognic strain JS／95／03 and respiratory strain SD／97／01 of infectious bronchitis virus were amplified，cloned and sequenced The nucleotide and deduced amino acid sequences of S1 genes from two isolates and ten reference strains were compared． The results showed that JS／95／03 and SD／97／01 were geneticalty closely related to M41 and H120．respectively． So jt speculated that they were possible variants of raceine strains Two isolates were different in pathogenicity．but they genetically related．Only 24 amino acid differences were found between S1 glycoproteins of two isolates， and 15 of them occurred in the region of the first 130 amino acid residues A amino acid differece Rt the position 1 I6 might he involved in pathDgenicity The corctpa~一 son of the predicted secondary structures of S1 glycoproteins from two isolates revealed that only one or a few amino acid differences were r~ponsible for different antigenicity and structure．
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        沈阳化工大学材料科学与工程学院 沈阳 110142

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      Am plification，Cloning and Sequence Analysis of S1 Genes from Infectious Bronchitis Virus Isolates JS／95／03 and SD／97／01

      • 1. College of Veterinary Medicine．Nanjing agriculture University，Nanjing 210095-China
      • 2. Institute of Agrlculturecie~i~es in Jiangsu Coastal Area，Yancheng 224002．China

      Abstract: Abstract：The entire S1 genes from nephropathognic strain JS／95／03 and respiratory strain SD／97／01 of infectious bronchitis virus were amplified，cloned and sequenced The nucleotide and deduced amino acid sequences of S1 genes from two isolates and ten reference strains were compared． The results showed that JS／95／03 and SD／97／01 were geneticalty closely related to M41 and H120．respectively． So jt speculated that they were possible variants of raceine strains Two isolates were different in pathogenicity．but they genetically related．Only 24 amino acid differences were found between S1 glycoproteins of two isolates， and 15 of them occurred in the region of the first 130 amino acid residues A amino acid differece Rt the position 1 I6 might he involved in pathDgenicity The corctpa~一 son of the predicted secondary structures of S1 glycoproteins from two isolates revealed that only one or a few amino acid differences were r~ponsible for different antigenicity and structure．

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