Isolation and Purification of the Eriocheir sinensis “Tremble Disease”Virus and Its Pathongenity on Crabs

SUN Xue-qiang; LU Cheng-ping

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April 2002

SUN Xue-qiang and LU Cheng-ping. Isolation and Purification of the Eriocheir sinensis “Tremble Disease”Virus and Its Pathongenity on Crabs[J]. Virologica Sinica, 2002, 17(2): 185-188.

Citation: SUN Xue-qiang, LU Cheng-ping. Isolation and Purification of the Eriocheir sinensis “Tremble Disease”Virus and Its Pathongenity on Crabs .VIROLOGICA SINICA, 2002, 17(2) : 185-188.

河蟹颤抖病病毒的分离纯化及其动物致病性试验

孙学强 ,
陆承平

1.
南京农业大学农业部动物疫病诊断与免疫重点开放实验室，南京2101395

摘要

特人工复制发病的河蟹(中华绒赘蟹)组织匀浆，离心上清经聚乙二醇(PEG 6 000)沉淀，再经分子筛层析．分部收集分别测定256-300am的吸光值。各收集峰嗣PEG20000，于4℃浓缩．制作负染标本经透射电镜观察，发现在第一峰浓缩液中存在大量球状病毒颗粒大小5O 100 。用纯化的病毒进行动物致病性试验，接种石蟹(锯齿华溪蟹)发生“颤抖病”，并用ELISA双抗体夹心法进行检测，显示感染石蟹较对照石蟹有明显差异。
- 河蟹
- , 颤抖病
- , 病毒

Isolation and Purification of the Eriocheir sinensis “Tremble Disease”Virus and Its Pathongenity on Crabs

1.
Kay lab of Aniatat Disease Diagnosis＆ tmmunotogy，Ministry of Agriculture，Nanjing Agri Univ，Nanjing 210095，China

Abstract

Abstract：The Eriocheir sinensis(Es)that suffered“tremble diease”by artificial infection were ho— mogenated The supernatant was concentrated with p。】yethyIeneg1ycol(PEG)6000 and purified by gel filtration chromatogmph with Sepia,arose 4B．The eluates collected were concentrated at 4℃ ．The virus particles in the first part of the eluates could be seen under electron microscopy．The diameter of virions varified jn 50～ 100nm Sinopotamon denticu缸￡“?II artilicia】inlected with the purified virus became j1]．Bv double antibody sandwich EI ISA detection．the results showed that there was signifi— cai]t diflerence between the jnfected and the centre crabs
- Eriocheir sinensis
- , Tremble disease
- , Virus

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Isolation and Purification of the Eriocheir sinensis “Tremble Disease”Virus and Its Pathongenity on Crabs

1. Kay lab of Aniatat Disease Diagnosis＆ tmmunotogy，Ministry of Agriculture，Nanjing Agri Univ，Nanjing 210095，China

Abstract: Abstract：The Eriocheir sinensis(Es)that suffered“tremble diease”by artificial infection were ho— mogenated The supernatant was concentrated with p。】yethyIeneg1ycol(PEG)6000 and purified by gel filtration chromatogmph with Sepia,arose 4B．The eluates collected were concentrated at 4℃ ．The virus particles in the first part of the eluates could be seen under electron microscopy．The diameter of virions varified jn 50～ 100nm Sinopotamon denticu缸￡“?II artilicia】inlected with the purified virus became j1]．Bv double antibody sandwich EI ISA detection．the results showed that there was signifi— cai]t diflerence between the jnfected and the centre crabs