Epitope M apping of Hepatitis C Virus NS3 Antigen from a Phage Peptide Library by Using Imm obilized Specific M onocionai Antibody

ZHONG Yan—wei; CHENG Jun; CHEN Xin—hua; WANG Gang; HONG Yuan; W ANG Lin; LI Li; ZHANG Ling—xia; CHEN Ju～mei

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June 2002

ZHONG Yan—wei, CHENG Jun, CHEN Xin—hua, WANG Gang, HONG Yuan, W ANG Lin, LI Li, ZHANG Ling—xia and CHEN Ju～mei. Epitope M apping of Hepatitis C Virus NS3 Antigen from a Phage Peptide Library by Using Imm obilized Specific M onocionai Antibody[J]. Virologica Sinica, 2002, 17(3): 202-205.

Citation: ZHONG Yan—wei, CHENG Jun, CHEN Xin—hua, WANG Gang, HONG Yuan, W ANG Lin, LI Li, ZHANG Ling—xia, CHEN Ju～mei. Epitope M apping of Hepatitis C Virus NS3 Antigen from a Phage Peptide Library by Using Imm obilized Specific M onocionai Antibody .VIROLOGICA SINICA, 2002, 17(3) : 202-205.

应用噬菌体随机肽库技术筛选丙肝病毒NS3抗原模拟表位

1.
解放军第三o二医院传染病研究所基因治疗研究中心、感染四科，北京100039

摘要

以抗．HCV NS3的单克隆抗体作为固相筛选分子，对人工合成的噬菌体随机12肽库进行5轮“吸附一洗脱一扩增”的筛选过程，随机挑取42个克隆，经噬菌体酶联免疫吸附法(ELISA)鉴定并进行交叉反应实验以及竞争抑制性结合实验，最后对所选克隆进行DNA序列分析，以确定HCV NS3抗原的模拟表位。经噬菌体富集后，从随机筛选的42个克隆中得到11个阳性克隆，确定氨基酸序列xxIxxxXMSNxx为HCV NS3的模拟表位。我们用噬菌体 l2肽库成功筛选得到HCV NS3的模拟表位，为开展用HCV 模拟表位探索HCV的防治研究创造了条件。
- 丙型肝炎病毒
- , 非结构蛋白NS3
- , 模拟表位
- , 噬菌体肽库

Epitope M apping of Hepatitis C Virus NS3 Antigen from a Phage Peptide Library by Using Imm obilized Specific M onocionai Antibody

1.
Gene Therapy Research Center，Institute of Infectious Diseseas ，Th 302 Hospital 0f PLA，Beijing 100039，China

Abstract

Using HCV NS3 m onoclonal antibody as selective molecule， a 12 m ar phage peptide library was biopanned and positive clones were selected by ELISA ． com petition assay and DN A sequencing． Eleven positive clones were chosen for DN A sequencing．From the experim ent and sequencing com par— ison results，one epitope was confirm ed as m imotope of HCV N S3．HCV N S3 m im otope was obtained by phage peptide library screening．The result provides a new approach for HCV therapy and vaccine developm ent．
- Hepatitis C virus
- , NS3
- , Mimotopes
- , Phage peptide library

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Epitope M apping of Hepatitis C Virus NS3 Antigen from a Phage Peptide Library by Using Imm obilized Specific M onocionai Antibody

1. Gene Therapy Research Center，Institute of Infectious Diseseas ，Th 302 Hospital 0f PLA，Beijing 100039，China

Abstract: Using HCV NS3 m onoclonal antibody as selective molecule， a 12 m ar phage peptide library was biopanned and positive clones were selected by ELISA ． com petition assay and DN A sequencing． Eleven positive clones were chosen for DN A sequencing．From the experim ent and sequencing com par— ison results，one epitope was confirm ed as m imotope of HCV N S3．HCV N S3 m im otope was obtained by phage peptide library screening．The result provides a new approach for HCV therapy and vaccine developm ent．