Sequence Analysis of Com plete L Segm ent of 84FLi， a Hantann Virus Isolated from Xi’an

Li Xin; YANG Wei; HANG Chang; BAI Xue; MA Ben; H UANG; LI Guang; ZHA NG

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June 2002

Li Xin, YANG Wei, HANG Chang, BAI Xue, MA Ben, H UANG, LI Guang and ZHA NG. Sequence Analysis of Com plete L Segm ent of 84FLi， a Hantann Virus Isolated from Xi’an[J]. Virologica Sinica, 2002, 17(3): 221-225.

Citation: Li Xin, YANG Wei, HANG Chang, BAI Xue, MA Ben, H UANG, LI Guang, ZHA NG. Sequence Analysis of Com plete L Segm ent of 84FLi， a Hantann Virus Isolated from Xi’an .VIROLOGICA SINICA, 2002, 17(3) : 221-225.

汉滩病毒西安分离株84FLi的L片段核苷酸序列测定以及分析

1.
1．第四军医大学唐都医院全军感染病中心，陕西西安710038
2.
中国预防医学科学院病毒学研究所。北京100052

摘要

采用逆转录一聚合酶链式反应(RT—PCR)，分节段扩增汉滩病毒84FLi株的L基因片段eDNA。纯化的PCR产物片段直接用于序列测定或克隆入pND18一T载体中进行测序。结果表明。84FLi株的L片段eDNA 由6533个核苷酸组成，四种核苷酸的比例分别为A33．39％，C16．43％。G20．74％，T29．44％。GC 含量为37．17％。AT 含量为 62．83％。推导出的最大开放读码框架为从38到6493，共编码2151个氨基酸。序列同源性分析表明，84FLi株核苷酸与HTN型国际标准毒株76—118的同源性为83．7％，差异性为18．8％；而与中国株A9的同源性高达97．6％。差异性仅为2．4％。与SEO型代表Seou180—39的同源性为75．2％。66．1％～66．5％。L片段的氨基酸比较分析表明，L片段与HTN型问的同源性为97．5％～98．0％，而与SEO型的同源性为83．5％～85．5％。与PUC、TUL、SN 和AND等其它型汉滩病毒的同源性仅为68．6％～69．6％。结果表明84FLi株属于HTN型，并与分离自国内的 HTN型病毒高度同源。
- 汉滩病毒84FLi株
- , L片段
- , 序列测定
- , 系统发生树

Sequence Analysis of Com plete L Segm ent of 84FLi， a Hantann Virus Isolated from Xi’an

1.
1．Infectious Diseases Center of P．L．A，Tangdu Ho tal，Fourth Military Medical University，Xi’an 710038，China
2.
Institute of Virology，Chinese Academy of Preventive Medicine，Beijing 100052。Ch ina

Abstract

In order tO study the nucleotide sequence of L segment of Hantaan virus strain 84FI i，the cDNA of L segment was amplified fragment by fragment by RT—PCR ．The purified PCR products were sequenced directly or cloned into pM D18一T vector and then sequenced．The I genom e segm ent is 6533 nucleotides in length with a predicated region(from 38 to 6493)encoding 2151 amino acids．The entire sequence com position was A 33．39％，C16．43％，G20．74％，T29．44％，and the GC and AT contents were 37．17 and 62．83％．Homology analysis showed that the homologies of 84FLi L segment nu— cleotide sequence with HTN isolates especially Chinese isolates were higher than those of other Han— tavirus．It showed 83．7％ homology with HTN foreign standard strain 76—118。while the amino acid homology between the two strains was 97．5％．The result suggested that 84FLi strain was one of the Hantann virus，and highly related tO other Chinese Hantaan Virus iso lates．
- Hantavirus
- , 84FLi strain
- , L segment
- , Sequence analysis

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Sequence Analysis of Com plete L Segm ent of 84FLi， a Hantann Virus Isolated from Xi’an

1. 1．Infectious Diseases Center of P．L．A，Tangdu Ho tal，Fourth Military Medical University，Xi’an 710038，China
2. Institute of Virology，Chinese Academy of Preventive Medicine，Beijing 100052。Ch ina

Abstract: In order tO study the nucleotide sequence of L segment of Hantaan virus strain 84FI i，the cDNA of L segment was amplified fragment by fragment by RT—PCR ．The purified PCR products were sequenced directly or cloned into pM D18一T vector and then sequenced．The I genom e segm ent is 6533 nucleotides in length with a predicated region(from 38 to 6493)encoding 2151 amino acids．The entire sequence com position was A 33．39％，C16．43％，G20．74％，T29．44％，and the GC and AT contents were 37．17 and 62．83％．Homology analysis showed that the homologies of 84FLi L segment nu— cleotide sequence with HTN isolates especially Chinese isolates were higher than those of other Han— tavirus．It showed 83．7％ homology with HTN foreign standard strain 76—118。while the amino acid homology between the two strains was 97．5％．The result suggested that 84FLi strain was one of the Hantann virus，and highly related tO other Chinese Hantaan Virus iso lates．