The Study on BIV Activity in Human Cells M T -4

WANG Shu—hui 。XIONG Kun; YANG Yi—shu; ZHU Yi—xin; XIA Qiu—yu; CHEN Guo-min; WANG Jin—zhong; CHEN Qi—min; GENG Yun—qi 一; ZENG Yi

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August 2002

WANG Shu—hui 。XIONG Kun, YANG Yi—shu, ZHU Yi—xin, XIA Qiu—yu, CHEN Guo-min, WANG Jin—zhong, CHEN Qi—min, GENG Yun—qi 一 and ZENG Yi. The Study on BIV Activity in Human Cells M T -4[J]. Virologica Sinica, 2002, 17(4): 354-357.

Citation: WANG Shu—hui 。XIONG Kun, YANG Yi—shu, ZHU Yi—xin, XIA Qiu—yu, CHEN Guo-min, WANG Jin—zhong, CHEN Qi—min, GENG Yun—qi 一, ZENG Yi. The Study on BIV Activity in Human Cells M T -4 .VIROLOGICA SINICA, 2002, 17(4) : 354-357.

BIV在人源细胞MT一4中的活性研究

1.
1．南开大学生命科学学院．天津300071：2．中国预防医学科学院病毒所．北京100052

摘要

牛免疫缺陷病毒(Bovine imm“” ciency virus，BIV)在分类上属于反转录病毒科的慢病毒属，目前尚未见 BIV感染人的报道。为进一步确定BIV对人源细胞的感染性，我们用BIVl27cDNA转染人源细胞MT一4，通过RT— PCR检测到BlVgag基因的转录，IFA则显示BIVl27的gag或gag-pol基因在MT一4细胞中得到了翻译，而RT值的测定也有力地说明BIVl27(-DNA已经在MT一4细胞内表达出有活性的反转录酶，但细胞传代实验表明BIV不能在 MT一4细胞内复制。
- 牛免疫缺陷病毒：MT一4细胞
- , 基因表达
- , 感染

The Study on BIV Activity in Human Cells M T -4

1.
1．College ofLife Sciences，Nankai University，Tia， in 300071，China

Abstract

Bovine immunodeficiency virus(BIV)is a kind of Lentivirus belonging tO Retrovirus，and up tO now．there is no report about itS infection tO human．In order to check BIV S infection to human cells，we transfected BIVl27 cDAN into human cells MT一4．By RT～PCR，we checked the transcription of BIV S gag gene，and determined the expression of gag or gag-pol gene in M T一4 cell by IFA．Re— verse Transcriptase(RT)assay showed the reverse transcriptase of BIVl27 had been expressed，while cell infection experiment indicated that BIV couldn t replicate in MT一4 cells．
- Bovein immunodeficiency virus
- , MT一4 cells
- , Gene expression

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

The Study on BIV Activity in Human Cells M T -4

1. 1．College ofLife Sciences，Nankai University，Tia， in 300071，China

Abstract: Bovine immunodeficiency virus(BIV)is a kind of Lentivirus belonging tO Retrovirus，and up tO now．there is no report about itS infection tO human．In order to check BIV S infection to human cells，we transfected BIVl27 cDAN into human cells MT一4．By RT～PCR，we checked the transcription of BIV S gag gene，and determined the expression of gag or gag-pol gene in M T一4 cell by IFA．Re— verse Transcriptase(RT)assay showed the reverse transcriptase of BIVl27 had been expressed，while cell infection experiment indicated that BIV couldn t replicate in MT一4 cells．