Effect of Interaction Between BTV-Hbc and Different Species Cell and Charateristics of Group Specific Antigen

TANG Xing—san; DONG Chang—yuan’’; GUO Shu—fang; CHEN Xiao; CHEN Dong—e; GUI Yi—rui; LU Li—li; LUO Xiang

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August 2002

TANG Xing—san, DONG Chang—yuan’’, GUO Shu—fang, CHEN Xiao, CHEN Dong—e, GUI Yi—rui, LU Li—li and LUO Xiang. Effect of Interaction Between BTV-Hbc and Different Species Cell and Charateristics of Group Specific Antigen[J]. Virologica Sinica, 2002, 17(4): 362-366.

Citation: TANG Xing—san, DONG Chang—yuan’’, GUO Shu—fang, CHEN Xiao, CHEN Dong—e, GUI Yi—rui, LU Li—li, LUO Xiang. Effect of Interaction Between BTV-Hbc and Different Species Cell and Charateristics of Group Specific Antigen .VIROLOGICA SINICA, 2002, 17(4) : 362-366.

蓝舌病毒HbC株与不同种系细胞相互作用及群特异性抗原特征

1.
武汉大学医学院病毒研究所肿瘤病毒研究室，湖北武汉，430071

摘要

：BTV．HbC株和蓝舌病毒标准株BTV一10分别接种在不同种系细胞如猴肾传代细胞(Vero)、人宫颈癌细胞 (Hela)和小鼠神经胶质瘤细胞(C6)等细胞株上，比较研究了BTV．HbC在不同种系细胞上的增殖特征，BTV．HbC与 BTV．10在相同细胞上的复制增殖特征，病毒与细胞相互作用的显微和超微结构特征。用免疫交叉反应研究了 BTv—HbC株与BTV．10型标准株之间的血清学关系。本研究结合本室对BTv．HbC株基因组图谱分析和蓝舌病毒群特异性抗原编码基因的RT．PCR分析，进一步证实了BTV—HbC株可能是一个新的血清型蓝舌病毒。
- 蓝舌病毒
- , 病毒与细胞相互作用
- , BTV HbC株血清学特征

Effect of Interaction Between BTV-Hbc and Different Species Cell and Charateristics of Group Specific Antigen

1.
Institute of Virology，Medical College，Wuhan University，Wuhan 430071，China

Abstract

Bluetongue virus(BTV)HbC strain and BTV 10(the international standard strain)were respectively inoculated onto the different species monolayer cells such as Vero cell(monkey renal cel1)， C6 cell(mouse neuroglioma cel1)and Hela cell(human cervical cancer cel1)．We studied comparatively the replicated characteristics of BTV—HbC in different species cells，with BTV 10 in the same cells， morphologic characteristics under microscopy and electron microscopy after interaction between BTV— HbC and different cells．the serologic relation between BTV—HbC and BTV一10．It is further proved that the BTV—HbC strain is probably a new type of Bluetongue virus when connecting our previous re— search about BTV—HbC genome map and RT—PCR analyses of BTV—HbC gene coding group speci fic antigen．
- Bluetongue virus
- , Interaction between virus and cell
- , Serologic Characteristic of BTV—HbC

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Effect of Interaction Between BTV-Hbc and Different Species Cell and Charateristics of Group Specific Antigen

1. Institute of Virology，Medical College，Wuhan University，Wuhan 430071，China

Abstract: Bluetongue virus(BTV)HbC strain and BTV 10(the international standard strain)were respectively inoculated onto the different species monolayer cells such as Vero cell(monkey renal cel1)， C6 cell(mouse neuroglioma cel1)and Hela cell(human cervical cancer cel1)．We studied comparatively the replicated characteristics of BTV—HbC in different species cells，with BTV 10 in the same cells， morphologic characteristics under microscopy and electron microscopy after interaction between BTV— HbC and different cells．the serologic relation between BTV—HbC and BTV一10．It is further proved that the BTV—HbC strain is probably a new type of Bluetongue virus when connecting our previous re— search about BTV—HbC genome map and RT—PCR analyses of BTV—HbC gene coding group speci fic antigen．