Detection of Wheat yellow m osaic virus by Heter0gene0us Animal Double—antibody Sandwich ELIS：A

GENG Bo; HAN Cheng-gui”; ZHAI Ya-feng; WANG Hui-qing; YU Jia—lin; LIU Yi

PDF
Cite
Share

facebook

twitter

google

linkedin

February 2003

GENG Bo, HAN Cheng-gui”, ZHAI Ya-feng, WANG Hui-qing, YU Jia—lin and LIU Yi. Detection of Wheat yellow m osaic virus by Heter0gene0us Animal Double—antibody Sandwich ELIS：A[J]. Virologica Sinica, 2003, 18(1): 76-78.

Citation: GENG Bo, HAN Cheng-gui”, ZHAI Ya-feng, WANG Hui-qing, YU Jia—lin, LIU Yi. Detection of Wheat yellow m osaic virus by Heter0gene0us Animal Double—antibody Sandwich ELIS：A .VIROLOGICA SINICA, 2003, 18(1) : 76-78.

利用异种动物抗血清双抗夹心法检测小麦黄花叶病毒

1.
中国农业大学农业生物技术国家实验室与植物病理系，北京100094

摘要

Abstract：With purified Wheat yellow mosaic vires(WYMV) particles as antigen， cavy was injected to produce specific antiserum．Its titer was tested as Y1 28 by the method of micro-immunoprecipitation． Using cavy antiserum as capture antibody for trapping the virus and rabbit antiserum as detection antibody， the experiment system of heterogeneous animal DAS-ELISA (HADAS—ELISA)was established to detect WYMV．The system has the advantage of better specificity and sensitivity of detection．This method was proved to be successful and prattical in the detection of the virus in field—-collected sam-· ples， indicating that the experiment system of HADAS—ELTSA is superior to the indirect ELISA with rabbit antiserum only．
- Wheat yellow mosaic virus
- , Cavy antibody
- , Heterogeneous animal double—antibody
- , Sandwich ELISA

Detection of Wheat yellow m osaic virus by Heter0gene0us Animal Double—antibody Sandwich ELIS：A

1.
f State key Laboratoryfor A grobiotechnology and Department ofPlant Pathology，China Agricultural University Beijing 100094，China

Abstract

Abstract：With purified Wheat yellow mosaic vires(WYMV) particles as antigen， cavy was injected to produce specific antiserum．Its titer was tested as Y1 28 by the method of micro-immunoprecipitation． Using cavy antiserum as capture antibody for trapping the virus and rabbit antiserum as detection antibody， the experiment system of heterogeneous animal DAS-ELISA (HADAS—ELISA)was established to detect WYMV．The system has the advantage of better specificity and sensitivity of detection．This method was proved to be successful and prattical in the detection of the virus in field—-collected sam-· ples， indicating that the experiment system of HADAS—ELTSA is superior to the indirect ELISA with rabbit antiserum only．
- Wheat yellow mosaic virus
- , Cavy antibody
- , Heterogeneous animal double—antibody
- , Sandwich ELISA

References
Proportional views

PDF

Article Metrics

Article views(5066) PDF downloads(1090) Cited by(0)

Proportional views

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Detection of Wheat yellow m osaic virus by Heter0gene0us Animal Double—antibody Sandwich ELIS：A

1. f State key Laboratoryfor A grobiotechnology and Department ofPlant Pathology，China Agricultural University Beijing 100094，China

Abstract: Abstract：With purified Wheat yellow mosaic vires(WYMV) particles as antigen， cavy was injected to produce specific antiserum．Its titer was tested as Y1 28 by the method of micro-immunoprecipitation． Using cavy antiserum as capture antibody for trapping the virus and rabbit antiserum as detection antibody， the experiment system of heterogeneous animal DAS-ELISA (HADAS—ELISA)was established to detect WYMV．The system has the advantage of better specificity and sensitivity of detection．This method was proved to be successful and prattical in the detection of the virus in field—-collected sam-· ples， indicating that the experiment system of HADAS—ELTSA is superior to the indirect ELISA with rabbit antiserum only．