SU Xiao-yun, M IAO, XU Lihua, CHEN Desheng and CHEN Puyan. Expression and purification of HCLV E2 protein A,D antigenic domains and its preliminary application*[J]. Virologica Sinica, 2003, 18(2): 164-168.
Citation:
SU Xiao-yun, M IAO, XU Lihua, CHEN Desheng, CHEN Puyan.
Expression and purification of HCLV E2 protein A,D antigenic domains and its preliminary application* .VIROLOGICA SINICA, 2003, 18(2)
: 164-168.
猪瘟兔化弱毒E2蛋白A,D区的高效表达和蛋白纯化及其应用
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摘要
摘要:本试验对表达猪瘟E2囊膜糖蛋白的原核表达载体pET-E2的表达条件进行了优化,在此基础上,对表达的 蛋白质进行了纯化。在6 mol/L盐酸胍存在的条件下,将包涵体溶解在rifs.HCI中,并直接用于His Band亲和层 析。收集过柱产物并透析,在2%SDS条件下,对所得的重组蛋白质进行了定量。重组蛋白质被用来包被96孔 ELISA板并应用于免疫猪瘟抗体水平的测定。
Expression and purification of HCLV E2 protein A,D antigenic domains and its preliminary application*
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1.
I Key lab ofAnimal Diagnostic and Immunology,Ministry ofAgriculture,Nanjing Agricultural University,Nanfing 210095,China
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Abstract
Abstract:In this experiment the conditions for the expressing of HCLV E2 glycoprotein A and D an tigenic domains in E coli were optimized.On this basis,the expressed protein was purified by dissolving the inclusion body in Tris.HC1 with 6 mol/L guan dinine an d passed throh a His Bind affinity chromatography.The product was collected an d th e concentrated recombinan t protein was quentified. Th e recombinan t protein was then used to coat 96一well ELISA plates an d applied to detect the titers of the serums from swine immunized with HCLV vaccine.
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References
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Proportional views
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