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Volume 18 Issue 5
October 2003

    YANG Yu．ying。, YIE Jian．qiang, ZHAO Zhen—hua。, QIN Ai-jian and GU Yu．fang。. YANG Yuying。 ， YIE Jianqiang ，ZHAO Zhen—hua。 ， QIN Ai-jian ，GU Yufang[J]. Virologica Sinica, 2003, 18(5): 454-458.
    Citation: YANG Yu．ying。, YIE Jian．qiang, ZHAO Zhen—hua。, QIN Ai-jian, GU Yu．fang。. YANG Yuying。 ， YIE Jianqiang ，ZHAO Zhen—hua。 ， QIN Ai-jian ，GU Yufang .VIROLOGICA SINICA, 2003, 18(5) : 454-458.
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    禽白血病病毒J亚群内蒙株的分离与鉴定

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      1．内蒙古农业大学动物科学与医学学院，内蒙古呼和浩特，010018：2 扬州大学动物医学系，江苏扬州，225009

    • 摘要：本研究从曾见典型禽骨髓性白血病(Myeloid Leukosis，ML)病例的内蒙古某肉种鸡场随机选取的淘汰肉种 鸡中，分离出一株J亚群禽白血病病毒(Avian leukosis virus Subgroup J，AL、，_J)。利用PCR和间接免疫荧光反应 进行鉴定，J亚群禽白血病病毒内蒙株可以被两对ALV_J特异性引物扩增(特异条带约2．2kb和545bp)：且在特 异性单抗的间接免疫荧光检测中呈现强阳性荧光反应。此外，对山东一例肉种鸡骨髓性白血病病例亦进行了J亚 群禽白血病病毒的分离与鉴定。2．2kb PCR扩增物的测序结果表明，两株分离病毒的同源性为96．9％，与已分离 的SD9901和YZ9901株ALV_J同源性达94．2％,-05．4％。

    YANG Yuying。 ， YIE Jianqiang ，ZHAO Zhen—hua。 ， QIN Ai-jian ，GU Yufang

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      Inner Mongolia University ofAgriculture．Huhhot010018，China

    • Abstract：A strain of Avian leukosis virus(ALV．J1 subgroup J was isolated from an adult broiler breeder folck．which had a history of myeloid leukosis(ML)．in Inner Mongolian．Moreover,another strain of ALV J was isolated from a ML case in Shandong．The two isolates were positive reaction in polymerase chain reactions with two pairs of primers specific for ALV-J and gave antigenically strong reaction in the indirect fluorescence antibody assay(IFA)with ALV-J specific monoclonal antibody JE9． The sequences of the 2．2kb PCR products of the tWO strains had 96．9％ homology,an d had 94．2％ — 95．4％ homology to strain SD9901 and YZ9901 AL＼LJ．
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      YANG Yuying。 ， YIE Jianqiang ，ZHAO Zhen—hua。 ， QIN Ai-jian ，GU Yufang

      • 1. Inner Mongolia University ofAgriculture．Huhhot010018，China

      Abstract: Abstract：A strain of Avian leukosis virus(ALV．J1 subgroup J was isolated from an adult broiler breeder folck．which had a history of myeloid leukosis(ML)．in Inner Mongolian．Moreover,another strain of ALV J was isolated from a ML case in Shandong．The two isolates were positive reaction in polymerase chain reactions with two pairs of primers specific for ALV-J and gave antigenically strong reaction in the indirect fluorescence antibody assay(IFA)with ALV-J specific monoclonal antibody JE9． The sequences of the 2．2kb PCR products of the tWO strains had 96．9％ homology,an d had 94．2％ — 95．4％ homology to strain SD9901 and YZ9901 AL＼LJ．

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