Isolation and Identification of Swine hlfIuenza Virus

WU Rui; JIN Mei—fin; CHEN Huan-chuen; WANG Guei-hua; JIANG Zeng-hai

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December 2003

WU Rui, JIN Mei—fin, CHEN Huan-chuen, WANG Guei-hua and JIANG Zeng-hai. Isolation and Identification of Swine hlfIuenza Virus[J]. Virologica Sinica, 2003, 18(6): 553-556.

Citation: WU Rui, JIN Mei—fin, CHEN Huan-chuen, WANG Guei-hua, JIANG Zeng-hai. Isolation and Identification of Swine hlfIuenza Virus .VIROLOGICA SINICA, 2003, 18(6) : 553-556.

猪流感病毒分离鉴定

1.
华中农业大学畜牧兽医学院动物病毒室，湖北武汉，430070

摘要

用非免疫鸡胚从我国上海、福建、湖北分离到3株猪流感病毒，分另lJ命名为SSHI、SFJI和SHBI。SIV 分离株第5代病毒液对0．7％豚鼠红细胞的血凝活性分别1：2 、1：2 和1：2。，与H 亚型标准血清血凝抑制价1： 2。、1：2’和1：2 ，但不能被鸡新城疫阳性血清所抑制。纯化的病毒在电镜下观察，可见到猪流感典型的病毒粒子。病毒液接种于小自鼠，可表现出临床症状，剖杀后可观察到病毒性肺炎。猪体回归试验，可表现出临床症状和病理变化，从肺脏中可分离到病毒并且RT-PCR也可检测到病毒相应的基因片段。从纯化的病毒中提取RNA，进行RT．PCR，可扩增出预期的条带。 Abstract：Three H3 subtype strains of Swine influenza virus(SlV)，which were named SSH1，SFJ1 andSHB1，were isolated from Shanghai，Fujian and Hubei．These isolates could grow and replicate inchicken emb~o，and the hemagglutinin(HA)values were 1：2 ，1：2 and 1：2 ，respectively．Thehemagglutinin inhibitor(HI)titers of H subtype antiserum were 1：2 ，1：2 and 1：2。，respectively,whereas they were negative in HI assays with Newcastle disease an tiserum．Under electron micrograph，the virions showed various shapes．The mice infected witl1 allantoic fluid could arise syndromes andpathology；SIV could be isolated again and amplified by RT-PCR from the lung．RNA Was extractedfrom SIV which Was purified from infected allantoic fluid，an d expected ban d Was observed viaRT—PCR．

Isolation and Identification of Swine hlfIuenza Virus

1.
Animal Virus Lab, Animal Science and Veterinary College，HuazhongAgricultural University,Wuhan4307~ China

Abstract

Abstract：Three H3 subtype strains of Swine influenza virus(SlV)，which were named SSH1，SFJ1 and SHB1，were isolated from Shanghai，Fujian and Hubei．These isolates could grow and replicate in chicken emb~o，and the hemagglutinin(HA)values were 1：2 ，1：2 and 1：2 ，respectively．The hemagglutinin inhibitor(HI)titers of H subtype antiserum were 1：2 ，1：2 and 1：2。，respectively, whereas they were negative in HI assays with Newcastle disease an tiserum．Under electron micrograph， the virions showed various shapes．The mice infected witl1 allantoic fluid could arise syndromes an d pathology；SIV could be isolated again and amplified by RT-PCR from the lung．RNA Was extracted from SIV which Was purified from infected allantoic fluid，an d expected ban d Was observed via RT—PCR．
- Swine influenza virus
- , Isolation and Identification

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Isolation and Identification of Swine hlfIuenza Virus

1. Animal Virus Lab, Animal Science and Veterinary College，HuazhongAgricultural University,Wuhan4307~ China

Abstract: Abstract：Three H3 subtype strains of Swine influenza virus(SlV)，which were named SSH1，SFJ1 and SHB1，were isolated from Shanghai，Fujian and Hubei．These isolates could grow and replicate in chicken emb~o，and the hemagglutinin(HA)values were 1：2 ，1：2 and 1：2 ，respectively．The hemagglutinin inhibitor(HI)titers of H subtype antiserum were 1：2 ，1：2 and 1：2。，respectively, whereas they were negative in HI assays with Newcastle disease an tiserum．Under electron micrograph， the virions showed various shapes．The mice infected witl1 allantoic fluid could arise syndromes an d pathology；SIV could be isolated again and amplified by RT-PCR from the lung．RNA Was extracted from SIV which Was purified from infected allantoic fluid，an d expected ban d Was observed via RT—PCR．