Isolation and Identification of Type A Swine Influenza Virus from Shandong Province and Sequence Analysis of HA Gene

XU Chuan-tian; FAN Wei-xing*; ZHAO Hong-kun

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February 2004

XU Chuan-tian, FAN Wei-xing* and ZHAO Hong-kun. Isolation and Identification of Type A Swine Influenza Virus from Shandong Province and Sequence Analysis of HA Gene[J]. Virologica Sinica, 2004, 19(1): 27-31.

Citation: XU Chuan-tian, FAN Wei-xing*, ZHAO Hong-kun. Isolation and Identification of Type A Swine Influenza Virus from Shandong Province and Sequence Analysis of HA Gene .VIROLOGICA SINICA, 2004, 19(1) : 27-31.

A型猪流感病毒山东分离株鉴定及其HA基因序列分析

1.
山东农业大学动物科技学院，山东泰安 271018

摘要

从山东各地疑似流感发病猪分离到10株流感病毒，经国家流感中心鉴定均为A型流感病毒H9N2亚型。将其中一株Sw/SD/1/2003（H9N2）的血凝素全基因（HA）进行克隆与测序，与GenBank收录的其它猪流感和禽流感H9N2 亚型的HA基因进行比较，发现Sw/SD/1/2003（H9N2）的血凝素基因在核苷酸序列方面同广西1999年分离的禽流感毒株Ck/GX/99（H9N2）和2000年云南分离的禽流感毒株Ck/YN/2000(H9N2)的同源性最高；进化树分析表明Sw/SD/1/2003 (H9N2) 起源于禽源的H9N2亚型流感病毒；Sw/SD/1/2003 的HA氨基酸裂解位点与其他H9N2亚型不同，Sw/SD/1/2003 的HA氨基酸裂解位点是R-S-L-R-G, 而其它猪流感和禽流感H9N2亚型都是R-S-S-R-G
- 猪流感病毒
- , Sw/SD/1/2003（H9N2）
- , HA
- , 氨基酸裂解位点

Isolation and Identification of Type A Swine Influenza Virus from Shandong Province and Sequence Analysis of HA Gene

1.
College of Animal Science, Shandong Agricultural University, Taian 271018, China

Abstract

10 influenza viruses were isolated from sicked pigs in different places of Shandong province from autumn of 2002 to spring of 2003. They were all identified as Influenza A virus of H9N2 subtype by National Influenza Research Center. The complete HA gene of one isolate Sw/SD/1/2003 (H9N2) was cloned and sequenced and compared with other H9N2 isolates of avian and swine in GenBank. The results indicated that the isolate Sw/SD/1/2003 (H9N2) was similar to Ck/GX/1999 (H9N2) and Ck/YN/2000 (H9N2 ) in HA gene; Sw/SD/1/2003 might come from an avian influenza H9N2 subtype according to the phylogenetic analysis of HA genes; the amino acid sequence at the HA cleavage site in Sw/SD/1/2003 is R-S-L-R-G, but the sequences of all other H9N2 subtypes of porcine isolates and avian isolates are R-S-S-R-G.
- Swine influenza virus
- , Sw/SD/1/2003
- , HA
- , Amino acid cleave site

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Isolation and Identification of Type A Swine Influenza Virus from Shandong Province and Sequence Analysis of HA Gene

1. College of Animal Science, Shandong Agricultural University, Taian 271018, China

Abstract: 10 influenza viruses were isolated from sicked pigs in different places of Shandong province from autumn of 2002 to spring of 2003. They were all identified as Influenza A virus of H9N2 subtype by National Influenza Research Center. The complete HA gene of one isolate Sw/SD/1/2003 (H9N2) was cloned and sequenced and compared with other H9N2 isolates of avian and swine in GenBank. The results indicated that the isolate Sw/SD/1/2003 (H9N2) was similar to Ck/GX/1999 (H9N2) and Ck/YN/2000 (H9N2 ) in HA gene; Sw/SD/1/2003 might come from an avian influenza H9N2 subtype according to the phylogenetic analysis of HA genes; the amino acid sequence at the HA cleavage site in Sw/SD/1/2003 is R-S-L-R-G, but the sequences of all other H9N2 subtypes of porcine isolates and avian isolates are R-S-S-R-G.