Property of Viral Nucleic Acid from the Virus Causing the Trembling disease  in Eriocheir sinensis

JIN Ye; LU Cheng-ping; LI Xian

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February 2004

JIN Ye, LU Cheng-ping and LI Xian. Property of Viral Nucleic Acid from the Virus Causing the Trembling disease in Eriocheir sinensis[J]. Virologica Sinica, 2004, 19(1): 36-38.

Citation: JIN Ye, LU Cheng-ping, LI Xian. Property of Viral Nucleic Acid from the Virus Causing the Trembling disease in Eriocheir sinensis .VIROLOGICA SINICA, 2004, 19(1) : 36-38.

引致河蟹颤抖病的病毒的核酸定性*

陆承平 ¹ ,

1.
1. 南京农业大学农业部动物疫病诊断与免疫重点实验室, 江苏南京 210095
2.
江苏省疾病预防控制中心, 江苏南京 210009

摘要

对河蟹（中华绒螯蟹）“颤抖病”病毒及其核酸进行了电镜观察。纯化病毒经负染后由电镜观察显示，病毒粒子呈球状，有囊膜和纤突，直径约为150nm。纯化病毒经核酸酶降解杂核酸后，加入8mol/L尿素释放病毒核酸，经水相法展开后，用覆有碳膜的铜网取样，再经染色和真空镀膜，于电镜下观察。病毒核酸呈单股线状，底片经光学精确放大后，测定了核酸分子的长度，根据经验公式计算出病毒核酸分子量为5.05×106。抽提的病毒核酸在0.8%琼脂糖凝胶电泳中呈单一条带，大小为15~16kb，经核酸酶酶切显示，核酸对DNase I不敏感，对RNase、Mung Bean Nuclease敏感，根据以上特性判断，该核酸为ssRNA。
- 中华绒螯蟹
- , 颤抖病病毒
- , 核酸
- , 电镜观察

Property of Viral Nucleic Acid from the Virus Causing the Trembling disease in Eriocheir sinensis

JIN Ye ¹ ,
LU Cheng-ping ^1*,, ,
LI Xian ²

1.
1. Key Lab of Animal Disease Diagnostic and Immunology, Ministry of Agriculture, Nanjing Agricultural University, Nanjing 210095, China
2.
Jiangsu Province Center for Disease Prevention and Control, Nanjing 210009, China

Corresponding author: LU Cheng-ping,

Abstract

In this paper we reported the electron microscope(EM) examination of “Trembling disease” virus in Eriocheir sinensis. The virions were globular, with envelope and spikes, and about 150nm of diameter. The purified nucleic acid was treated with 8mol/L urea and the viral nucleic acid was transferred to the EM grids by aqueous technique. The viral nucleic acid was single stranded under EM observation. The molecular weight of the viral nucleic was calculated to be about 5.05×106. The agrosegel electrophoresis of the purified viral nucleic acid showed only one band, with the size of about 15~16kb. The nucleic acid was sensitive to RNase and Mung Bean Nuclease treatment, but not sensitive to Dnase I. The above data demonstrated that the nucleic acid should be a ssRNA.
- Eriocheir sinensis
- , Trembling disease virus
- , Nucleic Acid
- , Electronic Microscope

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Property of Viral Nucleic Acid from the Virus Causing the Trembling disease in Eriocheir sinensis

Corresponding author: LU Cheng-ping,

1. 1. Key Lab of Animal Disease Diagnostic and Immunology, Ministry of Agriculture, Nanjing Agricultural University, Nanjing 210095, China
2. Jiangsu Province Center for Disease Prevention and Control, Nanjing 210009, China

Abstract: In this paper we reported the electron microscope(EM) examination of “Trembling disease” virus in Eriocheir sinensis. The virions were globular, with envelope and spikes, and about 150nm of diameter. The purified nucleic acid was treated with 8mol/L urea and the viral nucleic acid was transferred to the EM grids by aqueous technique. The viral nucleic acid was single stranded under EM observation. The molecular weight of the viral nucleic was calculated to be about 5.05×106. The agrosegel electrophoresis of the purified viral nucleic acid showed only one band, with the size of about 15~16kb. The nucleic acid was sensitive to RNase and Mung Bean Nuclease treatment, but not sensitive to Dnase I. The above data demonstrated that the nucleic acid should be a ssRNA.