NIU Guo-dong and ZHANG Zhong-xin*. Interaction between Ubiquitin and IAP2 or IAP3 of Spodptera exigua Nucleopolyhedrovirus[J]. Virologica Sinica, 2004, 19(1): 49-53.
Citation:
NIU Guo-dong, ZHANG Zhong-xin*.
Interaction between Ubiquitin and IAP2 or IAP3 of Spodptera exigua Nucleopolyhedrovirus .VIROLOGICA SINICA, 2004, 19(1)
: 49-53.
利用双杂交系统对SeNPV泛素与抗细胞凋亡 蛋白相互作用的研究*
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摘要
本文报道利用酵母双杂交系统研究甜菜夜蛾核多角体病毒（SeNPV）的泛素（Ubiqutin）与抗细胞凋亡蛋白（IAP2, IAP3）相互作用的结果。使用Clontech 公司的MACHMAKER GAL4 Two-hybrid system 3，以病毒ubiquitin基因与酵母GAL4的DNA结合域重组表达“诱饵”蛋白，以病毒iap2或iap3基因与DNA活化域重组表达“猎物”蛋白，在低严谨型筛选培养基上均得到阳性克隆。这一结果表明，甜菜夜蛾核多角体病毒泛素与IAP2或IAP3在体外能进行相互作用，这种作用利用了酵母内源性E1和E2。SeNPV的抗细胞凋亡蛋白（IAPs）可能是泛素-蛋白酶水解途径（UPP）中的泛素连接酶（E3），或者是泛素依赖性蛋白水解酶的靶底物。
Interaction between Ubiquitin and IAP2 or IAP3 of Spodptera exigua Nucleopolyhedrovirus
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Key Laboratory of Molecular Virology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071, China
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Abstract
By using the yeast two hybrid system, in vitro assay for interaction between ubiquitin and IAP2 or IAP3 encoded by Spodptera exigua nucleopolyhedrovirus（SeNPV）has been done. The putative positive clones only existed on the plate of the SD/-Leu/-Trp/X-α-GAL, which suggested that ubiquitin interacted with IAP2 or IAP3 weakly, and ubiquitin was transferred to IAP2 or IAP3 through ubiquitin-activating enzyme (E1) and ubiquitin-conjugating enzyme (E2) in yeast. IAPs encoded by SeNPV may be the ubiquitin ligase (E3).
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References
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Proportional views
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