Study on inactivation of Tobacco mosaic virus by y3 from  Mushroom (Coprinus comatus)

WU Li-ping; WU Zu-jian; LIN Qi-ying*; XIE Lian-hui

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February 2004

WU Li-ping, WU Zu-jian, LIN Qi-ying* and XIE Lian-hui. Study on inactivation of Tobacco mosaic virus by y3 from Mushroom (Coprinus comatus)[J]. Virologica Sinica, 2004, 19(1): 54-57.

Citation: WU Li-ping, WU Zu-jian, LIN Qi-ying*, XIE Lian-hui. Study on inactivation of Tobacco mosaic virus by y3 from Mushroom (Coprinus comatus) .VIROLOGICA SINICA, 2004, 19(1) : 54-57.

一种食用菌提取物y3对烟草花叶病毒的钝化作用及其机制*

1.
福建农林大学植物病毒研究所, 福建福州 350002

摘要

测定了从食用菌毛头鬼伞（Coprinus comatus）中提纯的y3蛋白对烟草花叶病毒（Tobacco mosaic virus,TMV）的钝化作用，结果表明y3对TMV有较强的体外钝化作用，在心叶烟枯斑寄主上对TMV的抑制中浓度约为2.0μg/mL；y3在pH9.0时较稳定；TMV与y3混合后用RNase处理，测得侵染率为61.74%,比未用RNase处理的对照降低了38.26%，说明y3具有一定的体外脱衣壳作用；另外电镜观察发现y3可使部分TMV粒体发生裂解，变短。
- 烟草花叶病毒
- , 钝化

Study on inactivation of Tobacco mosaic virus by y3 from Mushroom (Coprinus comatus)

1.
Institute of Plant Virology, Fujian Agriculture and Forestry University, Fuzhou 350002, China

Abstract

A antiviral protein y3, from mushroom Coprinus comatus, could inactivate Tobacco mosaic virus (TMV) in vitro. The inhibition rate against is 50.0% when the concentration of y3 is 2.0μg/mL. When RNase was added to the mixture of TMV and y3, the infection rate of TMV was 61.74%, decreased by 38.26% in contrast with the control. It was also shown that y3 can break TMV particles when observed under electron microscope.
- Tobacco mosaic virus
- , Inactivation

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Study on inactivation of Tobacco mosaic virus by y3 from Mushroom (Coprinus comatus)

1. Institute of Plant Virology, Fujian Agriculture and Forestry University, Fuzhou 350002, China

Abstract: A antiviral protein y3, from mushroom Coprinus comatus, could inactivate Tobacco mosaic virus (TMV) in vitro. The inhibition rate against is 50.0% when the concentration of y3 is 2.0μg/mL. When RNase was added to the mixture of TMV and y3, the infection rate of TMV was 61.74%, decreased by 38.26% in contrast with the control. It was also shown that y3 can break TMV particles when observed under electron microscope.