Analysis of HIV Genotypes Isolated from Blood Donors in Beijing

SONG Ai-jing; LI Xiou-hua; ZHANG Chun-tao; WANG You-chun*

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October 2004

SONG Ai-jing, LI Xiou-hua, ZHANG Chun-tao and WANG You-chun*. Analysis of HIV Genotypes Isolated from Blood Donors in Beijing[J]. Virologica Sinica, 2004, 19(5): 517-519.

Citation: SONG Ai-jing, LI Xiou-hua, ZHANG Chun-tao, WANG You-chun*. Analysis of HIV Genotypes Isolated from Blood Donors in Beijing .VIROLOGICA SINICA, 2004, 19(5) : 517-519.

北京地区部分人类免疫缺陷病毒基因型分析

1.
中国药品生物制品检定所细胞室，北京 100050

摘要
- 人类免疫缺陷病毒
- , 克隆
- , 测序
- , 基因型

Analysis of HIV Genotypes Isolated from Blood Donors in Beijing

1.
Department of Cell Biology, National Institute for the Control of Pharmaceutical and Biological Products, Beijing 100050, China

Abstract

To investigate HIV genotypes isolated from blood donors in Beijing, the samples positive for anti-HIV antibody with ELISA were confirmed by HIV blot. All 26 samples were confirmed positive for anti-HIV antibody. RNA from those samples were detected with RT-PCR. 20 of them were positive for HIV RNA. The purified PCR products were cloned. The sequences were determined and analyzed. The results showed that 10 of 20 isolates were CRF BC, 9 of them were genotype B, 1 of them were CRF AE. 6 of 9 genotype B belong to B’(Thailand B), the remaining 3 isolates may belong to western genotype B. The results indicated that several different genotypes of HIV which are predominant in China may exist in Beijing. This result may be helpful for the development of HIV vaccine in Beijing.
- Human immunodeficiency viruses(HIV)
- , Cloning
- , Sequencing
- , Genotype

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Analysis of HIV Genotypes Isolated from Blood Donors in Beijing

1. Department of Cell Biology, National Institute for the Control of Pharmaceutical and Biological Products, Beijing 100050, China

Abstract: To investigate HIV genotypes isolated from blood donors in Beijing, the samples positive for anti-HIV antibody with ELISA were confirmed by HIV blot. All 26 samples were confirmed positive for anti-HIV antibody. RNA from those samples were detected with RT-PCR. 20 of them were positive for HIV RNA. The purified PCR products were cloned. The sequences were determined and analyzed. The results showed that 10 of 20 isolates were CRF BC, 9 of them were genotype B, 1 of them were CRF AE. 6 of 9 genotype B belong to B’(Thailand B), the remaining 3 isolates may belong to western genotype B. The results indicated that several different genotypes of HIV which are predominant in China may exist in Beijing. This result may be helpful for the development of HIV vaccine in Beijing.