Chemokine Genes Modulate Immune Responses Induced by HIV-1 Envelope Protein Gene Vaccine

WANG Fu-xiang; PAN Yan; SUN Yong-tao

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December 2005

WANG Fu-xiang, PAN Yan and SUN Yong-tao. Chemokine Genes Modulate Immune Responses Induced by HIV-1 Envelope Protein Gene Vaccine[J]. Virologica Sinica, 2005, 20(6): 578-581.

Citation: WANG Fu-xiang, PAN Yan, SUN Yong-tao. Chemokine Genes Modulate Immune Responses Induced by HIV-1 Envelope Protein Gene Vaccine .VIROLOGICA SINICA, 2005, 20(6) : 578-581.

趋化因子基因对HIV-1外膜蛋白基因疫苗诱导的免疫应答的影响

王福祥 ^1* ,
潘艳 ¹ ,
孙永涛 ²

1.
1.哈尔滨医科大学附属第一医院感染科黑龙江哈尔滨 2.第四军医大学唐都医院传染科陕西西安

摘要

研究趋化因子基因对HIV-1外膜蛋白基因疫苗诱导免疫应答的影响,以探求防治HIV的新策略。将 pVAX1GP120联合RANTES、MIP-1α基因或者pVAX1GP120单独免疫Balb／c小鼠,采用ELISA检测免疫小鼠的特异性抗体和IFN-γ水平,用MTT比色法检测免疫小鼠脾淋巴细胞的增殖,用乳酸脱氢酶(LDH)试验检测小鼠特异性细胞毒性T淋巴细胞(CTL)的应答。与pVAX1GP120免疫组比较,pVAX1GP120联合RANTES、MIP- 1α基因免疫组小鼠血清的抗HIV-1gp120抗体滴度升高,有显著性差异(p0．01);与pVAX1GP120免疫组比较, pVAX1GP120联合RANTES、MIP-1α基因免疫组小鼠血清的IFN-γ升高,有显著性差异(p0．01); pVAX1GP120联合RANTES、MIP-1α基因免疫组小鼠的脾淋巴细胞增殖实验刺激指数(SI)以及特异性CTL活性均高于pVAX1GP120免疫组,有显著性差异(p0．01)。RANTES、MIP-1α基因联合HIV-1外膜蛋白基因疫苗免疫小鼠,可能增强HIV特异性Th1细胞和CTL反应,RANTES、MIP-1α基因对体液免疫有加强作用。因此, RANTES、MIP-1α基因对于HIV-1外膜蛋白基因疫苗具有较好应用前景的免疫佐剂。
- HIV-1
- , 外膜蛋白基因疫苗
- , 免疫
- , RANTES
- , MIP-1α

Chemokine Genes Modulate Immune Responses Induced by HIV-1 Envelope Protein Gene Vaccine

Abstract

To investigate the effect of chemokine gene immunization on immune responses induced by HIV-1 envelope protein gene vaccine and to explore new strategies against HIV, Balb/c mice were immunized with pVAX1GP120 alone or co-administered with the DNA encoding for RANTES、and MIP-1α. Their sera were collected for analyzing anti-HIV antibody and IFN-γ by ELISA, and splenocytes of Balb/c mice were isolated for detecting antigen-specific lymphoprolif-erative responses and specific CTL response by MTT and LDH assays, respectively. Our results showed that the anti-HIV antibody tilers of mice co-immunized with pVAX1GP120 and the DNA encoding for RANTES and MIP-1α were higher than that of mice immunized with pVAX1GP120 alone (P0. 01). The IFN-γ level of mice co-immunized with pVAX1GP120 and the DNA encoding for RANTES and MIP-1α was higher than that of mice immunized with pVAX1GP120 alone (P0. 01). In comparison with mice injected with pVAX1GP120 alone, the specific CTL cyto-toxity activity and antigen-specific lymphop
- HIV-1
- , Envelope protein gene vaccine
- , Immunization
- , RANTES
- , MIP-1a

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Chemokine Genes Modulate Immune Responses Induced by HIV-1 Envelope Protein Gene Vaccine

Abstract: To investigate the effect of chemokine gene immunization on immune responses induced by HIV-1 envelope protein gene vaccine and to explore new strategies against HIV, Balb/c mice were immunized with pVAX1GP120 alone or co-administered with the DNA encoding for RANTES、and MIP-1α. Their sera were collected for analyzing anti-HIV antibody and IFN-γ by ELISA, and splenocytes of Balb/c mice were isolated for detecting antigen-specific lymphoprolif-erative responses and specific CTL response by MTT and LDH assays, respectively. Our results showed that the anti-HIV antibody tilers of mice co-immunized with pVAX1GP120 and the DNA encoding for RANTES and MIP-1α were higher than that of mice immunized with pVAX1GP120 alone (P0. 01). The IFN-γ level of mice co-immunized with pVAX1GP120 and the DNA encoding for RANTES and MIP-1α was higher than that of mice immunized with pVAX1GP120 alone (P0. 01). In comparison with mice injected with pVAX1GP120 alone, the specific CTL cyto-toxity activity and antigen-specific lymphop