Expression and Purification of Nucleocaspid and Envelope Proteins of SARS Coronavirus

HE Tao; HU Zhi-hong; FANG Ming-gang; WANG Hua-lin; YANG Ji-hong; WANG Han-zhong

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December 2005

HE Tao, HU Zhi-hong, FANG Ming-gang, WANG Hua-lin, YANG Ji-hong and WANG Han-zhong. Expression and Purification of Nucleocaspid and Envelope Proteins of SARS Coronavirus[J]. Virologica Sinica, 2005, 20(6): 590-593.

Citation: HE Tao, HU Zhi-hong, FANG Ming-gang, WANG Hua-lin, YANG Ji-hong, WANG Han-zhong. Expression and Purification of Nucleocaspid and Envelope Proteins of SARS Coronavirus .VIROLOGICA SINICA, 2005, 20(6) : 590-593.

SARS病毒N蛋白、E蛋白在大肠杆菌中的表达与鉴定

何涛 ^1,2 ,
胡志红 ¹ ,
方明刚 ¹ ,
王华林 ¹ ,
杨继红 ^1,3 ,
王汉中 ^1*

1.
1.湖北武汉中国科学院武汉病毒研究所病毒学国家重点实验室 2.中国科学院研究生院北京 3.华中师范大学生命科学院

摘要

本研究通过RT-PCR反应获得了SARS冠状病毒核衣壳蛋白(N)和膜蛋白(E)基因,将n基因和e基因克隆到大肠杆菌表达载体pGEX-KG上,并在大肠杆菌中以可溶形式获得高效表达,表达产物经亲和层析纯化。重组蛋白N与SARS病毒抗体呈现特异性的反应,为进一步研究SARS病毒感染免疫应答机制和早期诊断奠定基础
- SARS冠状病毒（SARS-Cov）
- , N蛋白
- , E蛋白
- , 表达

Expression and Purification of Nucleocaspid and Envelope Proteins of SARS Coronavirus

Abstract

Genes encoding nucleocapsid (N) and envelope (E) proteins of SARS coronavirus (SARS-CoV) were obtained by RT-PCR and were cloned into the expression vector pGEX-KG. The genes were expressed in E. coli and the recombinant proteins were in a soluble form. The proteins were purified by affinity chromatography. The recombinant N protein reacted specifically with anti-SARS antibody. The results may provide a good tool for further research of immune responses and early diagnosis of SARS virus.
- SARS Coronavirus（SARS-Cov）
- , Nucleocaspid protein
- , Envelope protein
- , Prokaryotic expression

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Expression and Purification of Nucleocaspid and Envelope Proteins of SARS Coronavirus

Abstract: Genes encoding nucleocapsid (N) and envelope (E) proteins of SARS coronavirus (SARS-CoV) were obtained by RT-PCR and were cloned into the expression vector pGEX-KG. The genes were expressed in E. coli and the recombinant proteins were in a soluble form. The proteins were purified by affinity chromatography. The recombinant N protein reacted specifically with anti-SARS antibody. The results may provide a good tool for further research of immune responses and early diagnosis of SARS virus.