Genetic and Immunogenic Characteristics of Measles Virus Isolated in Zhejiang Province

YAN Ju-ying; LU Yi-yu; ZHANG Jian-hua; FEN Yan; SHI Wen; MAO Hai-yan; GONG Li-ming; GE Qiong; ZHOU Min; LI Min-hong

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February 2006

YAN Ju-ying, LU Yi-yu, ZHANG Jian-hua, FEN Yan, SHI Wen, MAO Hai-yan, GONG Li-ming, GE Qiong, ZHOU Min and LI Min-hong. Genetic and Immunogenic Characteristics of Measles Virus Isolated in Zhejiang Province[J]. Virologica Sinica, 2006, 21(1): 1-5.

Citation: YAN Ju-ying, LU Yi-yu, ZHANG Jian-hua, FEN Yan, SHI Wen, MAO Hai-yan, GONG Li-ming, GE Qiong, ZHOU Min, LI Min-hong. Genetic and Immunogenic Characteristics of Measles Virus Isolated in Zhejiang Province .VIROLOGICA SINICA, 2006, 21(1) : 1-5.

浙江省麻疹病毒分离株的基因特性与免疫原性研究

1.
浙江省疾病预防控制中心，浙江杭州 310009

通讯作者： 严菊英, ;

摘要

为了研究浙江省1999～2003年麻疹病毒分离株的抗原性变化以及基因特性,阐明其基因特性与麻疹流行的关系,以及抗原性变化对疫苗免疫效果的影响。我们从麻疹患者含漱液中分离麻疹病毒株,制备大鼠免疫血清,与疫苗株沪191和Edmonston株等进行交叉中和试验,测定各毒株之间抗原比;并采集儿童麻疹疫苗免疫前后血清,分别测定其对不同毒株的中和抗体滴度;采用RT-PCR法扩增麻疹毒株血凝素蛋白(H)及核蛋白(N)基因,进行核苷酸序列测定。结果浙江99-1和浙江02-2株与沪191株之间的抗原比分别为3.0和7.3;儿童免疫后血清对浙江02-2株的麻疹中和抗体平均几何滴度(GMT)为15.03,明显低于沪191疫苗株(GMT为68.12);浙江省1999～2003年麻疹分离株之间,H基因氨基酸同源性为99.8%,N基因氨基酸同源性为98.5%～100.0%;与沪191疫苗株的H基因和N基因相比较,分离株与其在氨基酸水平上同源性分别为95.5%～95.7%和95.9%～96.3%。从基因进化树显示,1999～2003年浙江省分离到的麻疹毒株属于H1基因型,但与疫苗株沪191在抗原性和基因特性上已存在明显的差异。
- 麻疹病毒
- , 沪191疫苗株
- , 抗原性
- , 基因特性

Genetic and Immunogenic Characteristics of Measles Virus Isolated in Zhejiang Province

1.
Zhejiang Center for Disease Control and Prevention, Hangzhou 310009, China

Corresponding author: YAN Ju-ying,

Abstract

In order to elucidate the relationship between genetic characteristics and measles outbreak,and the influence of antigenic variation on measles vaccine effect,we investigated the genetic characteristics and antigenic variation of measles viruses isolated in Zhejiang province during 1999～ 2003.Rats were immunized with the measles virus isolated from patients and the titers of the antibody were measured by cross neutralization test using the antigen of the isolated measles virus,vaccine strains of Shanghai191...
- Measles viruses
- , Shanghai-191 vaccine strain
- , Antigenicity
- , Genetic characteristics

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Genetic and Immunogenic Characteristics of Measles Virus Isolated in Zhejiang Province

Corresponding author: YAN Ju-ying,

1. Zhejiang Center for Disease Control and Prevention, Hangzhou 310009, China

Abstract: In order to elucidate the relationship between genetic characteristics and measles outbreak,and the influence of antigenic variation on measles vaccine effect,we investigated the genetic characteristics and antigenic variation of measles viruses isolated in Zhejiang province during 1999～ 2003.Rats were immunized with the measles virus isolated from patients and the titers of the antibody were measured by cross neutralization test using the antigen of the isolated measles virus,vaccine strains of Shanghai191...