Characterization of Spike Gene of Infectious Bronchitis Coronavirus CK/CH/ LDL97Ⅰ/97 Isolated in China

LIU Xin; LIU Sheng-wang; KONG Xian-gang; ZHANG Qing-xia; RONG Jun-gong; HAN Zong-xi; SHAO Yu-hao

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February 2006

LIU Xin, LIU Sheng-wang, KONG Xian-gang, ZHANG Qing-xia, RONG Jun-gong, HAN Zong-xi and SHAO Yu-hao. Characterization of Spike Gene of Infectious Bronchitis Coronavirus CK/CH/ LDL97Ⅰ/97 Isolated in China[J]. Virologica Sinica, 2006, 21(1): 68-70.

Citation: LIU Xin, LIU Sheng-wang, KONG Xian-gang, ZHANG Qing-xia, RONG Jun-gong, HAN Zong-xi, SHAO Yu-hao. Characterization of Spike Gene of Infectious Bronchitis Coronavirus CK/CH/ LDL97Ⅰ/97 Isolated in China .VIROLOGICA SINICA, 2006, 21(1) : 68-70.

鸡传染性支气管炎病毒CK/CH/LDL97Ⅰ/97株S基因特征

刘昕 ^1,2 ,
刘胜旺 ^1,, ,
孔宪刚 ¹ ,
张庆霞 ¹ ,
荣骏弓 ¹ ,
韩宗玺 ¹ ,
邵昱昊 ¹

1.
1. 中国农业科学院哈尔滨兽医研究所兽医生物技术国家重点实验室，黑龙江哈尔滨 150001
2.
新疆农业大学动物医学学院，新疆乌鲁木齐 830052

通讯作者： 刘胜旺, ;

摘要
- 传染性支气管炎病毒
- , S糖蛋白
- , 基因特征

Characterization of Spike Gene of Infectious Bronchitis Coronavirus CK/CH/ LDL97Ⅰ/97 Isolated in China

1.
1. National Key Laboratory of Veterinary Biotechnology, Institute of Harbin Veterinary Medicine, CAAS, Harbin 150001, China
2.
College of Veterinary Medicine, Xinjiang Agricultural University, Urumqi 830052, China

Corresponding author: LIU Sheng-wang,

Abstract

The spike gene (S) of Infectious bronchitis virus (IBV) CK/CH/LDL97Ⅰ/97 isolate was amplified，cloned, sequenced and analyzed. Results showed that the S gene was 3501 bp encoding a polypeptide of 1167 amino acids. The precursor of the S protein was cleaved into S1 and S2 fragments, which comprised 541 and 626 amino acid residues, respectively. The cleavage site sequence was RRTGR. The homologies of nucleotide and amino acid sequences of S1 gene between CK/CH/LDL97Ⅰ/97 and 27 reference IBV strains ranged from 60.6% to 99.6%, and 50.5% to 99.1%, respectively. The CK/CH/LDL97Ⅰ/97 S1 was most similar to that of three proventriculus IBV isolates, Q1(99.1%), J2(98.9%), T3 (98.9%), which were isolated in China. Phylogenetic analysis of S1 proteins indicated that CK/CH/LDL97Ⅰ/97, Q1, J2 and T3 formed a separated cluster, which might belong to a novel genotype of IBV.
- Infectious bronchitis virus
- , Spike glycoprotein
- , Molecular characterization

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Characterization of Spike Gene of Infectious Bronchitis Coronavirus CK/CH/ LDL97Ⅰ/97 Isolated in China

Corresponding author: LIU Sheng-wang,

1. 1. National Key Laboratory of Veterinary Biotechnology, Institute of Harbin Veterinary Medicine, CAAS, Harbin 150001, China
2. College of Veterinary Medicine, Xinjiang Agricultural University, Urumqi 830052, China

Abstract: The spike gene (S) of Infectious bronchitis virus (IBV) CK/CH/LDL97Ⅰ/97 isolate was amplified，cloned, sequenced and analyzed. Results showed that the S gene was 3501 bp encoding a polypeptide of 1167 amino acids. The precursor of the S protein was cleaved into S1 and S2 fragments, which comprised 541 and 626 amino acid residues, respectively. The cleavage site sequence was RRTGR. The homologies of nucleotide and amino acid sequences of S1 gene between CK/CH/LDL97Ⅰ/97 and 27 reference IBV strains ranged from 60.6% to 99.6%, and 50.5% to 99.1%, respectively. The CK/CH/LDL97Ⅰ/97 S1 was most similar to that of three proventriculus IBV isolates, Q1(99.1%), J2(98.9%), T3 (98.9%), which were isolated in China. Phylogenetic analysis of S1 proteins indicated that CK/CH/LDL97Ⅰ/97, Q1, J2 and T3 formed a separated cluster, which might belong to a novel genotype of IBV.