JDV Tat has Strong Transactivate Ability Because of its RNA Binding Domain

DENG Gang; SU Yang; MU Jun-jie; LI Yue; QIAO Wen-tao; GENG Yun-qi; CHEN Qi-min

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April 2006

DENG Gang, SU Yang, MU Jun-jie, LI Yue, QIAO Wen-tao, GENG Yun-qi and CHEN Qi-min. JDV Tat has Strong Transactivate Ability Because of its RNA Binding Domain[J]. Virologica Sinica, 2006, 21(2): 142-147.

Citation: DENG Gang, SU Yang, MU Jun-jie, LI Yue, QIAO Wen-tao, GENG Yun-qi, CHEN Qi-min. JDV Tat has Strong Transactivate Ability Because of its RNA Binding Domain .VIROLOGICA SINICA, 2006, 21(2) : 142-147.

RNA结合域决定JDV Tat具有较强的激活能力

1.
南开大学生命科学学院，天津 300071

通讯作者： 陈启民*,

摘要

为分析JDV与BIV、HIV-1 LTR和Tat相互激活能力差异的原因，在氨基酸序列对比及HIV-1 Tat功能域划分的基础上构建了JH、HJ、JB、BJ几种嵌合Tat蛋白，并克隆到真核表达载体。将上述表达质粒与以JDV、BIV和HIV-1 LTR为启动子，以luc为报告基因的质粒共转染Hela细胞，证实了三种不同Tat激活能力的差异主要来自其结合域RNA结合能力的差异，排除了结构域不完整和细胞因子缺乏造成JH不激活HIV-1 LTR的可能性。
- JDV
- , HIV-1
- , BIV
- , LTR
- , 嵌合蛋白

JDV Tat has Strong Transactivate Ability Because of its RNA Binding Domain

1.
College of Life Sciences, Nankai University, Tianjin 300071, China

Corresponding author: CHEN Qi-min,

Abstract

In order to find the cause of different transactivate abilities among JDV, BIV and HIV-1 Tat, four chimeric Tat cDNA expression constructs, JH, HJ, JB and BJ, were generated with the crossover points at the boundary of activation and binding domains based on functional domain division of HIV-1 Tat and amino acid sequence comparision among HIV-1, JDV and BIV Tat. These chimeric Tat proteins were co-transfected with JDV, BIV and HIV-1 LTR report plasmids in Hela. Transient assay showed that different transactivate abilities among JDV, BIV and HIV-1 Tat were mainly caused by different binding abilities of their binding domains. We further excluded some possibilities that may cause the poor transactivate ability of JH on HIV-1 LTR, such as incomplete functional domains and the lack of related cytokines.
- JDV
- , HIV-1
- , BIV
- , LTR
- , Fusion protein

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

JDV Tat has Strong Transactivate Ability Because of its RNA Binding Domain

Corresponding author: CHEN Qi-min,

1. College of Life Sciences, Nankai University, Tianjin 300071, China

Abstract: In order to find the cause of different transactivate abilities among JDV, BIV and HIV-1 Tat, four chimeric Tat cDNA expression constructs, JH, HJ, JB and BJ, were generated with the crossover points at the boundary of activation and binding domains based on functional domain division of HIV-1 Tat and amino acid sequence comparision among HIV-1, JDV and BIV Tat. These chimeric Tat proteins were co-transfected with JDV, BIV and HIV-1 LTR report plasmids in Hela. Transient assay showed that different transactivate abilities among JDV, BIV and HIV-1 Tat were mainly caused by different binding abilities of their binding domains. We further excluded some possibilities that may cause the poor transactivate ability of JH on HIV-1 LTR, such as incomplete functional domains and the lack of related cytokines.